Germline transcription from T-cell receptor Vbeta gene is uncoupled from allelic exclusion.

Published

Journal Article

Allelic exclusion operates in B and T lymphocytes to ensure clonal expression of antigen receptors after V(D)J recombination. Germline transcription, which proceeds V(D)J recombination, has been postulated to provide an instructive signal for allelic exclusion. Here, we use a genetic marker to track germline transcription from a Vbeta gene within the TCRbeta locus. We find that developing thymocytes exhibit uniformed, bi-allelic activation of the Vbeta gene before V-DJ recombination, a process subject to allelic exclusion. We further show that V-DJ rearrangement promotes activation rather than silencing of germline transcription from the remaining Vbeta genes on either the functionally or non-functionally rearranged chromosome. Results presented here suggest that germline transcription, although necessary for V(D)J recombination, is not sufficient to instruct allelic exclusion.

Full Text

Duke Authors

Cited Authors

  • Jia, J; Kondo, M; Zhuang, Y

Published Date

  • May 2007

Published In

Volume / Issue

  • 26 / 9

Start / End Page

  • 2387 - 2399

PubMed ID

  • 17410206

Pubmed Central ID

  • 17410206

Electronic International Standard Serial Number (EISSN)

  • 1460-2075

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1038/sj.emboj.7601671

Language

  • eng