Therapeutic B cell depletion impairs adaptive and autoreactive CD4+ T cell activation in mice.

Published

Journal Article

CD20 antibody depletion of B lymphocytes effectively ameliorates multiple T cell-mediated autoimmune diseases through mechanisms that remain unclear. To address this, a mouse CD20 antibody that depletes >95% of mature B cells in mice with otherwise intact immune systems was used to assess the role of B cells in CD4(+) and CD8(+) T cell activation and expansion in vivo. B cell depletion had no direct effect on T cell subsets or the activation status of CD4(+) and CD8(+) T cells in naive mice. However, B cell depletion impaired CD4(+) T cell activation and clonal expansion in response to protein antigens and pathogen challenge, whereas CD8(+) T cell activation was not affected. In vivo dendritic cell ablation, along with CD20 immunotherapy, revealed that optimal antigen-specific CD4(+) T cell priming required both B cells and dendritic cells. Most importantly, B cell depletion inhibited antigen-specific CD4(+) T cell expansion in both collagen-induced arthritis and autoimmune diabetes mouse models. These results provide direct evidence that B cells contribute to T cell activation and expansion in vivo and offer insights into the mechanism of action for B cell depletion therapy in the treatment of autoimmunity.

Full Text

Duke Authors

Cited Authors

  • Bouaziz, J-D; Yanaba, K; Venturi, GM; Wang, Y; Tisch, RM; Poe, JC; Tedder, TF

Published Date

  • December 26, 2007

Published In

Volume / Issue

  • 104 / 52

Start / End Page

  • 20878 - 20883

PubMed ID

  • 18093919

Pubmed Central ID

  • 18093919

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0709205105

Language

  • eng

Conference Location

  • United States