Effects of 9,12,15-octadecatrien-6-ynoic acid on the metabolism of arachidonic acid in platelets and on the platelet aggregation.

Published

Journal Article

An acetylenic fatty acid: 9,12,15-octadecatrien-6-ynoic acid (dicranin), extracted from Dicranum Scoparium was preincubated with platelets stimulated by exogenous arachidonic acid (20:4 n-6). Dicranin (10(-4) M) weakly inhibited the cyclooxygenase activity as assessed by measurement of 12-hydroxy-heptadecatrienoic acid (HHT) In contrast, the 12-hydroxy-eicosatetraenoic acid (12-HETE) synthesized by the 12-lipoxygenase was strongly increased by about 650%. The same effects were observed with 10(-6) M of dicranin but to a lesser extent. The main platelet hydroxylated dicranin metabolite determined by GC-MS was a 13-hydroxy derivative Platelet aggregation induced either by thrombin or by arachidonic acid or by U46619, an structural PGH2 analogue was inhibited by 10(-4) M of dicranin.

Full Text

Duke Authors

Cited Authors

  • Guichardant, M; Michel, M; Borel, C; Fay, L; Crozier, G; Magnolato, D; Finot, PA

Published Date

  • 1992

Published In

Volume / Issue

  • 37 /

Start / End Page

  • 215 - 221

PubMed ID

  • 1632297

Pubmed Central ID

  • 1632297

International Standard Serial Number (ISSN)

  • 0379-0363

Language

  • eng

Conference Location

  • Switzerland