Fibroblast growth factor receptor 2 regulates proliferation and Sertoli differentiation during male sex determination.

Journal Article

Targeted mutagenesis of Fgf9 in mice causes male-to-female sex reversal. Among the four FGF receptors, FGFR2 showed two highly specific patterns based on antibody staining, suggesting that it might be the receptor-mediating FGF9 signaling in the gonad. FGFR2 was detected at the plasma membrane in proliferating coelomic epithelial cells and in the nucleus in Sertoli progenitor cells. This expression pattern suggested that Fgfr2 might play more than one role in testis development. To test the hypothesis that Fgfr2 is required for male sex determination, we crossed mice carrying a floxed allele of Fgfr2 with two different Cre lines to induce a temporal or cell-specific deletion of this receptor. Results show that deletion of Fgfr2 in embryonic gonads phenocopies deletion of Fgf9 and leads to male-to-female sex reversal. Using these two Cre lines, we provide the first genetic evidence that Fgfr2 plays distinct roles in proliferation and Sertoli cell differentiation during testis development.

Full Text

Duke Authors

Cited Authors

  • Kim, Y; Bingham, N; Sekido, R; Parker, KL; Lovell-Badge, R; Capel, B

Published Date

  • October 16, 2007

Published In

Volume / Issue

  • 104 / 42

Start / End Page

  • 16558 - 16563

PubMed ID

  • 17940049

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0702581104

Language

  • eng

Conference Location

  • United States