Time and pressure dependence of transvascular Clara cell protein, albumin, and IgG transport during ventilator-induced lung injury in mice.

Journal Article

We compared the transport of three proteins with different hydrodynamic radii with ultrastructural changes in lungs of intact mice ventilated at peak inflation pressures (PIP) of 15, 35, 45, and 55 cmH(2)O for 2 h and PIP of 55 cmH(2)O for 0.5 and 1 h. After 2 h of ventilation, significant increases were observed in plasma Clara cell secretory protein (1.9 nm radius) at 35 cmH(2)O PIP and in bronchoalveolar lavage fluid albumin (3.6 nm radius) at 45 cmH(2)O PIP and IgG (5.6 nm radius) at 55 cmH(2)O PIP. Increased concentrations of all three proteins and lung wet-to-dry weight ratios were significantly correlated with PIP and ventilation time. Clara cell secretory protein and albumin increased significantly after 0.5 h of 55 cmH(2)O PIP, but IgG increased only after 2 h. Separation of endothelium or epithelium to form blebs was apparent only in small vessels (15-30 microm diameter) at 45 cmH(2)O PIP and after 0.5 h at 55 cmH(2)O PIP but became extensive after 2 h of ventilation at 55 cmH(2)O PIP. Junctional gaps between cells were rarely observed. Ultrastructural lung injury and protein clearances across the air-blood barrier were related to ventilation time and PIP levels. Protein clearances increased in relation to molecular size, consistent with increasing dimensions and frequency of transmembrane aqueous pathways.

Full Text

Duke Authors

Cited Authors

  • Yoshikawa, S; King, JA; Reynolds, SD; Stripp, BR; Parker, JC

Published Date

  • March 2004

Published In

Volume / Issue

  • 286 / 3

Start / End Page

  • L604 - L612

PubMed ID

  • 14761872

International Standard Serial Number (ISSN)

  • 1040-0605

Digital Object Identifier (DOI)

  • 10.1152/ajplung.00283.2003

Language

  • eng

Conference Location

  • United States