Human cystic fibrosis transmembrane conductance regulator directed to respiratory epithelial cells of transgenic mice.

Journal Article

Human cystic fibrosis transmembrane conductance regulator (CFTR) was expressed in transgenic mice under the control of transcriptional elements derived from the human surfactant protein C (SP-C) gene. The hCFTR mRNA was expressed in lungs and testes: in the lung, we found hCFTR mRNA in bronchiolar and alveolar epithelial cells, and CFTR protein in respiratory epithelial cells. While the level of expression of hCFTR mRNA varied, hCFTR mRNA and protein were detected in pulmonary epithelial cells of several lines. Lung weight, morphology, somatic growth and reproductive capacity were not altered by expression hCFTR in lung and testes of the transgenics. Our findings suggest that hCFTR can be safely transferred to lung epithelial cells for CF therapy.

Full Text

Duke Authors

Cited Authors

  • Whitsett, JA; Dey, CR; Stripp, BR; Wikenheiser, KA; Clark, JC; Wert, SE; Gregory, RJ; Smith, AE; Cohn, JA; Wilson, JM

Published Date

  • September 1992

Published In

Volume / Issue

  • 2 / 1

Start / End Page

  • 13 - 20

PubMed ID

  • 1284640

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng0992-13

Language

  • eng

Conference Location

  • United States