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Effect of 5-hydroxytryptamine1 serotonin receptor agonists on noxiously stimulated micturition in cats with chronic spinal cord injury.

Publication ,  Journal Article
Gu, B; Thor, KB; Reiter, JP; Dolber, PC
Published in: The Journal of urology
June 2007

The serotonin 5-hydroxytryptamine(1A/7) receptor agonist (R)-8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)tetralin) (Sigma) and the 5-hydroxytryptamine(1A/1B/1D) agonist GR-46611 (3-[3-(2-dimethylaminoethyl)-1H-indol-5-yl]-N-(4-methoxybenzyl)acrylamide) (Tocris Cookson, Ellisville, Missouri) inhibit bladder activity during saline infusion into the bladder of cats with chronic spinal cord injury (saline infused, spinal cord injured cats), suggesting an effect on mechanosensitive bladder afferent C fibers or their targets. We investigated the effects of (R)-8-OH-DPAT and GR-46611 on bladder activity in chronic spinal cord injured cats during infusion of dilute acetic acid into the bladder to stimulate chemosensitive bladder afferent C fibers (acid infused, spinal cord injured cats).Chloralose anesthetized, spinal cord injured cats were catheterized through the bladder dome for filling cystometry during 0.5% acetic acid infusion. Dose-response curves for (R)-8-OH-DPAT (0.3 to 30 microg/kg intravenously) or GR-46611 (0.03 to 300 microg/kg intravenously) were followed by the 5-hydroxytryptamine(1A) antagonist WAY-100635 (N-tert-butyl-3-(4-(2-methoxyphenyl)-piperazin-1-yl)-2-phenylpropanamide) (Sigma) (300 microg/kg). Threshold volume, bladder capacity, residual volume, micturition volume and arterial pressure were measured and external urethral sphincter electromyogram was recorded.Acid infused, spinal cord injured cats responded to (R)-8-OH-DPAT but not to GR-46611 with dose dependent increases in threshold volume, capacity and residual volume (significant above 3 microg/kg). Effects of (R)-8-OH-DPAT were largely reversed by WAY-100635. Neither (R)-8-OH-DPAT nor GR-46611 augmented external urethral sphincter electromyogram activity.Based on differences in the response to GR-46611 in saline vs acid infused, spinal cord injured animals it is tempting to speculate that 2 distinct populations of bladder afferent C fibers (1 chemosensitive and 1 mechanosensitive) can initiate spinal bladder reflexes. Because 5-hydroxytryptamine(1A) receptor agonists increased bladder capacity under saline or acid infused conditions, they are promising candidates for decreasing bladder hyperactivity and increasing bladder capacity in patients with chronic spinal cord injury.

Duke Scholars

Published In

The Journal of urology

DOI

EISSN

1527-3792

ISSN

0022-5347

Publication Date

June 2007

Volume

177

Issue

6

Start / End Page

2381 / 2385

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder, Neurogenic
  • Stimulation, Chemical
  • Spinal Cord Injuries
  • Serotonin Receptor Agonists
  • Nerve Fibers, Unmyelinated
  • Indoles
  • Female
  • Cats
  • Animals
 

Citation

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Gu, B., Thor, K. B., Reiter, J. P., & Dolber, P. C. (2007). Effect of 5-hydroxytryptamine1 serotonin receptor agonists on noxiously stimulated micturition in cats with chronic spinal cord injury. The Journal of Urology, 177(6), 2381–2385. https://doi.org/10.1016/j.juro.2007.01.110
Gu, Baojun, Karl B. Thor, Jerome P. Reiter, and Paul C. Dolber. “Effect of 5-hydroxytryptamine1 serotonin receptor agonists on noxiously stimulated micturition in cats with chronic spinal cord injury.The Journal of Urology 177, no. 6 (June 2007): 2381–85. https://doi.org/10.1016/j.juro.2007.01.110.
Gu, Baojun, et al. “Effect of 5-hydroxytryptamine1 serotonin receptor agonists on noxiously stimulated micturition in cats with chronic spinal cord injury.The Journal of Urology, vol. 177, no. 6, June 2007, pp. 2381–85. Epmc, doi:10.1016/j.juro.2007.01.110.
Gu B, Thor KB, Reiter JP, Dolber PC. Effect of 5-hydroxytryptamine1 serotonin receptor agonists on noxiously stimulated micturition in cats with chronic spinal cord injury. The Journal of urology. 2007 Jun;177(6):2381–2385.
Journal cover image

Published In

The Journal of urology

DOI

EISSN

1527-3792

ISSN

0022-5347

Publication Date

June 2007

Volume

177

Issue

6

Start / End Page

2381 / 2385

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder, Neurogenic
  • Stimulation, Chemical
  • Spinal Cord Injuries
  • Serotonin Receptor Agonists
  • Nerve Fibers, Unmyelinated
  • Indoles
  • Female
  • Cats
  • Animals