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Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells.

Publication ,  Journal Article
Wickstrom, EL; Bacon, TA; Gonzalez, A; Lyman, GH; Wickstrom, E
Published in: In Vitro Cell Dev Biol
March 1989

The proto-oncogene c-myc, whose gene product has a role in replication, is overexpressed in the human promyelocytic leukemia HL-60 cell line. Treatment of HL-60 cells with an antisense oligodeoxyribonucleotide complementary to the start codon and the next four codons of c-myc mRNA has previously been observed to inhibit c-myc protein expression and cell proliferation in a sequence-specific, dose-dependent manner. Comparable effects are seen upon treatment of HL-60 cells with dimethylsulfoxide (Me2SO), which is also known to induce granulocytic differentiation of HL-60 cells. Hence, the effects of antisense oligomers on cellular differentiation were examined and compared with Me2SO. Differentiation of HL-60 cells into forms with granulocytic characteristics was found to be enhanced in a sequence-specific manner by the anti-c-myc oligomer. No synergism was observed between the anti-c-myc oligomer and Me2SO in stimulating cellular differentiation. In contrast, synergism did appear in the inhibition of cell proliferation. Finally, the anti-c-myc oligomer uniformly inhibited colony formation in semisolid medium. It is possible that further reduction in the level of c-myc expression by antisense oligomer inhibition may be sufficient to allow terminal granulocytic differentiation and reverse transformation.

Duke Scholars

Published In

In Vitro Cell Dev Biol

DOI

ISSN

0883-8364

Publication Date

March 1989

Volume

25

Issue

3 Pt 1

Start / End Page

297 / 302

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Oxidation-Reduction
  • Oligonucleotides, Antisense
  • Oligonucleotides
  • Nitroblue Tetrazolium
  • Leukemia, Promyelocytic, Acute
 

Citation

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Wickstrom, E. L., Bacon, T. A., Gonzalez, A., Lyman, G. H., & Wickstrom, E. (1989). Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells. In Vitro Cell Dev Biol, 25(3 Pt 1), 297–302. https://doi.org/10.1007/BF02628470
Wickstrom, E. L., T. A. Bacon, A. Gonzalez, G. H. Lyman, and E. Wickstrom. “Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells.In Vitro Cell Dev Biol 25, no. 3 Pt 1 (March 1989): 297–302. https://doi.org/10.1007/BF02628470.
Wickstrom EL, Bacon TA, Gonzalez A, Lyman GH, Wickstrom E. Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells. In Vitro Cell Dev Biol. 1989 Mar;25(3 Pt 1):297–302.
Wickstrom, E. L., et al. “Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells.In Vitro Cell Dev Biol, vol. 25, no. 3 Pt 1, Mar. 1989, pp. 297–302. Pubmed, doi:10.1007/BF02628470.
Wickstrom EL, Bacon TA, Gonzalez A, Lyman GH, Wickstrom E. Anti-c-myc DNA increases differentiation and decreases colony formation by HL-60 cells. In Vitro Cell Dev Biol. 1989 Mar;25(3 Pt 1):297–302.

Published In

In Vitro Cell Dev Biol

DOI

ISSN

0883-8364

Publication Date

March 1989

Volume

25

Issue

3 Pt 1

Start / End Page

297 / 302

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Proto-Oncogene Proteins c-myc
  • Proto-Oncogene Proteins
  • Proto-Oncogene Mas
  • Oxidation-Reduction
  • Oligonucleotides, Antisense
  • Oligonucleotides
  • Nitroblue Tetrazolium
  • Leukemia, Promyelocytic, Acute