Comparison of commercial peanut skin test extracts.

Published

Journal Article

BACKGROUND:Skin prick testing is a major tool for diagnosing food allergy. Food allergen extracts have not been standardized; this may lead to great variability in the predictive accuracy of skin prick tests. METHODS:Six commercial peanut skin test extracts were compared in vitro with RAST inhibition assays, ELISA, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting with sera from peanut-allergic adults and in vivo by skin prick testing. RESULTS:ELISA showed that the content of peanut allergens Ara h I and Ara h II in the extracts ranged from 0.0015 to 0.0236 and 0.0001 to 0.0164 mg Eq/ml, respectively. RAST inhibition studies showed that the extracts produced curves of similar slope, suggesting conservation of allergenic epitopes. SDS-PAGE revealed differences in protein profiles because roasted extracts generally possessed the same number and proportion of major protein bands but raw extracts varied more in both respects. SDS-PAGE and immunoblotting showed that two of the extracts contained major IgE-binding protein bands that did not appear in the others. One roasted extract gave little protein banding and consequently little IgE binding. CONCLUSIONS:Skin testing results showed no differences in the ability of the extracts to provoke a positive skin test response in peanut-sensitive subjects.

Full Text

Cited Authors

  • Hefle, SL; Helm, RM; Burks, AW; Bush, RK

Published Date

  • April 1995

Published In

Volume / Issue

  • 95 / 4

Start / End Page

  • 837 - 842

PubMed ID

  • 7722164

Pubmed Central ID

  • 7722164

Electronic International Standard Serial Number (EISSN)

  • 1097-6825

International Standard Serial Number (ISSN)

  • 0091-6749

Digital Object Identifier (DOI)

  • 10.1016/s0091-6749(95)70127-3

Language

  • eng