Competitive inhibition ELISA for quantification of Ara h 1 and Ara h 2, the major allergens of peanuts.


Journal Article

Allergies to peanuts are becoming an increasingly important health problem as a result of the persistence and severity of the reaction in allergic individuals. Because no treatment currently is available, avoidance is the only option for peanut-allergic individuals. Avoidance of an abundant and often disguised food such as peanuts, however, is very difficult; therefore, competitive inhibition ELISAs were developed to detect and quantitate each of the major peanut allergens, Ara h 1 and Ara h 2. Under optimal conditions for each assay, the sensitivity of the Ara h 1 and Ara h 2 detection assays were 12 and 0.5 ng/mL, respectively. These assays were primarily devised to effectively compare the levels of Ara h 1 and Ara h 2 in a wide variety of peanuts or peanut products but can also be used to identify cross-reactive antigens. The method is simple and rapid, requiring only one allergen-specific antibody and, therefore, could be adapted specifically to detect the presence of these individual allergens in different foods.

Full Text

Cited Authors

  • Schmitt, DA; Cheng, H; Maleki, SJ; Burks, AW

Published Date

  • November 2004

Published In

Volume / Issue

  • 87 / 6

Start / End Page

  • 1492 - 1497

PubMed ID

  • 15675464

Pubmed Central ID

  • 15675464

Electronic International Standard Serial Number (EISSN)

  • 1944-7922

International Standard Serial Number (ISSN)

  • 1060-3271


  • eng