Isolation of the 36-kD German (Blattella germanica) cockroach allergen using fast protein liquid chromatography.

Published

Journal Article

Cockroach allergens have been established as important sensitizing agents in the induction of asthma. In the present investigation, we identified a 36-kD allergen from German cockroach (Blattella germanica) using fast protein liquid chromatography that reacted in the Bla g II monoclonal assay developed by Pollart et al. Defatted whole body German cockroaches were extracted in phosphate-buffered saline and fractionated using gel filtration (Superdex 75) and anion exchange (MonoQ) chromatography. The 36-kD allergen was isolated from this enriched allergen preparation by excision from modified preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels, electroelution, dialysis and lyophilization. SDS-PAGE/Western immunoblot analysis using a serum pool of cockroach-sensitive individuals revealed a single band. The lyophilized 36-kD allergen was then analyzed for amino acid composition and digested for peptide amino acid sequencing.

Full Text

Cited Authors

  • Helm, RM; Brenner, RJ; Williams, LW; Burks, AW

Published Date

  • January 1, 1994

Published In

Volume / Issue

  • 103 / 1

Start / End Page

  • 59 - 66

PubMed ID

  • 8260851

Pubmed Central ID

  • 8260851

Electronic International Standard Serial Number (EISSN)

  • 1423-0097

International Standard Serial Number (ISSN)

  • 1018-2438

Digital Object Identifier (DOI)

  • 10.1159/000236606

Language

  • eng