Identification of peanut agglutinin and soybean trypsin inhibitor as minor legume allergens.

Published

Journal Article

Peanuts and soybeans are frequent causes of food hypersensitivity reactions in children. Sera from 12 patients with atopic dermatitis and a positive double-blind placebo-controlled food challenge to peanut and sera from 5 patients with atopic dermatitis and a positive double-blind placebo-controlled food challenge to soybean were used to identify and characterize specific legume allergens. Identification of a minor allergen from peanut and a minor allergen from soybean was accomplished using various physicochemical techniques. The peanut fraction, peanut agglutinin, isolated by anion-exchange chromatography and electrolution and confirmed by amino acid sequencing, bound IgE in only 50% of the peanut challenge positive patients. The soybean fraction, soybean trypsin inhibitor, identified by gel filtration and electroelution and confirmed by amino acid sequencing, bound IgE in only 20% of the soy challenge positive patients. The identification of these two known legume proteins as minor allergens should allow further immunologic and structural investigations to compare the major and minor legume allergens.

Full Text

Cited Authors

  • Burks, AW; Cockrell, G; Connaughton, C; Guin, J; Allen, W; Helm, RM

Published Date

  • October 1994

Published In

Volume / Issue

  • 105 / 2

Start / End Page

  • 143 - 149

PubMed ID

  • 7920014

Pubmed Central ID

  • 7920014

Electronic International Standard Serial Number (EISSN)

  • 1423-0097

International Standard Serial Number (ISSN)

  • 1018-2438

Digital Object Identifier (DOI)

  • 10.1159/000236816

Language

  • eng