Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish.

Journal Article (Journal Article)

Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration.

Full Text

Duke Authors

Cited Authors

  • Yin, VP; Thomson, JM; Thummel, R; Hyde, DR; Hammond, SM; Poss, KD

Published Date

  • March 15, 2008

Published In

Volume / Issue

  • 22 / 6

Start / End Page

  • 728 - 733

PubMed ID

  • 18347091

Pubmed Central ID

  • PMC2275425

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.1641808


  • eng

Conference Location

  • United States