Methyl tert-butyl ether (MTBE) degradation by a microbial consortium.

Published

Journal Article

The widespread use of methyl tert-butyl ether (MTBE) as a gasoline additive has resulted in a large number of cases of groundwater contamination. Bioremediation is often proposed as the most promising alternative after treatment. However, MTBE biodegradation appears to be quite different from the biodegradation of usual gasoline contaminants such as benzene, toluene, ethyl benzene and xylene (BTEX). In the present paper, the characteristics of a consortium degrading MTBE in liquid cultures are presented and discussed. MTBE degradation rate was fast and followed zero order kinetics when added at 100 mg l(-1). The residual MTBE concentration in batch degradation experiments ranged from below the detection limit (1 microg l(-1)) to 50 microg l(-1). The specific activity of the consortium ranged from 7 to 52 mgMTBE g(dw)(-1) h(-1) (i.e. 19-141 mgCOD g(dw) (-1) h(-1)). Radioisotope experiments showed that 79% of the carbon-MTBE was converted to carbon-carbon dioxide. The consortium was also capable of degrading a variety of hydrocarbons, including tert-butyl alcohol (TBA), tert-amyl methyl ether (TAME) and gasoline constituents such as benzene, toluene, ethylbenzene and xylene (BTEX). The consortium was also characterized by a very slow growth rate (0.1 d(-1)), a low overall biomass yield (0.11 gdw g(-1)MTBE; i.e. 0.040 gdw gCOD(-1)), a high affinity for MTBE and a low affinity for oxygen, which may be a reason for the slow or absence of MTBE biodegradation in situ. Still, the results presented here show promising perspectives for engineering the in situ bioremediation of MTBE.

Full Text

Duke Authors

Cited Authors

  • Fortin, NY; Morales, M; Nakagawa, Y; Focht, DD; Deshusses, MA

Published Date

  • June 2001

Published In

Volume / Issue

  • 3 / 6

Start / End Page

  • 407 - 416

PubMed ID

  • 11472505

Pubmed Central ID

  • 11472505

Electronic International Standard Serial Number (EISSN)

  • 1462-2920

International Standard Serial Number (ISSN)

  • 1462-2912

Digital Object Identifier (DOI)

  • 10.1046/j.1462-2920.2001.00206.x

Language

  • eng