Ultrasound-induced cell detachment and gene transfection in adherent cells

Journal Article

The effect of 1-MHz cw ultrasound (0.36 MPa in pressure amplitude) on cell detachment and gene transfection in adherent HeLa cells was investigated. Cell detachment, cell injury, and gene transfection (up to 50%) in surviving populations were found to increase progressively with exposure duration (0-20 s). However, with respect to the total exposed cells, gene transfection efficiency in the adherent cells (3%) was significantly lower than its counterpart without detachment (15%). Cell detachment by ultrasound exposure was accompanied by significantly reduced proliferation rate with greatly increased apoptosis in the surviving detached cell population. © 2005 Acoustical Society of America.

Full Text

Duke Authors

Cited Authors

  • Lu, X; Zhong, P

Published Date

  • 2005

Published In

Volume / Issue

  • 6 / 3

Start / End Page

  • 195 - 200

International Standard Serial Number (ISSN)

  • 1529-7853

Digital Object Identifier (DOI)

  • 10.1121/1.1909243