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High intensity focused ultrasound-induced gene activation in solid tumors.

Publication ,  Journal Article
Liu, Y; Kon, T; Li, C; Zhong, P
Published in: J Acoust Soc Am
July 2006

In this work, the activation of heat-sensitive trans-gene by high-intensity focused ultrasound (HIFU) in a tumor model was investigated. 4T1 cancer cells (2 x 10(6)) were inoculated subcutaneously in the hind limbs of Balb/C mice. The tumors were subsequently transducted on day 10 by intratumoral injection of a heat-sensitive adenovirus vector (Adeno-hsp70B-Luc at 2 x 10(8) pfu/tumor). On day 11, the tumors were heated to a peak temperature of 55, 65, 75, or 85 degrees C within 10-30 s at multiple sites around the center of the tumor by a 1.1- or 3.3-MHz HIFU transducer. Inducible luciferase gene expression was increased from 15-fold to 120-fold of the control group following 1.1-MHz HIFU exposure. Maximum gene activation (120-fold) was produced at a peak temperature of 65-75 degrees C one day following HIFU exposure and decayed to baseline within 7 days. HIFU-induced gene activation (75 degrees C-10 s) could be further improved by using a 3.3-MHz transducer and a dense scan strategy to 170-fold. Thermal stress, rather than nonthermal mechanical stress, was identified as the primary physical mechanism for HIFU-induced gene activation in vivo. Overall, these observations open up the possibility for combining HIFU thermal ablation with heat-regulated gene therapy for cancer treatment.

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Published In

J Acoust Soc Am

DOI

ISSN

0001-4966

Publication Date

July 2006

Volume

120

Issue

1

Start / End Page

492 / 501

Location

United States

Related Subject Headings

  • Ultrasonography
  • Transcriptional Activation
  • Stress, Mechanical
  • Promoter Regions, Genetic
  • Neoplasms, Experimental
  • Mice, Inbred BALB C
  • Mice
  • Luminescent Agents
  • Luciferases
  • Hyperthermia, Induced
 

Citation

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Liu, Y., Kon, T., Li, C., & Zhong, P. (2006). High intensity focused ultrasound-induced gene activation in solid tumors. J Acoust Soc Am, 120(1), 492–501. https://doi.org/10.1121/1.2205129
Liu, Yunbo, Takashi Kon, Chuanyuan Li, and Pei Zhong. “High intensity focused ultrasound-induced gene activation in solid tumors.J Acoust Soc Am 120, no. 1 (July 2006): 492–501. https://doi.org/10.1121/1.2205129.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound-induced gene activation in solid tumors. J Acoust Soc Am. 2006 Jul;120(1):492–501.
Liu, Yunbo, et al. “High intensity focused ultrasound-induced gene activation in solid tumors.J Acoust Soc Am, vol. 120, no. 1, July 2006, pp. 492–501. Pubmed, doi:10.1121/1.2205129.
Liu Y, Kon T, Li C, Zhong P. High intensity focused ultrasound-induced gene activation in solid tumors. J Acoust Soc Am. 2006 Jul;120(1):492–501.

Published In

J Acoust Soc Am

DOI

ISSN

0001-4966

Publication Date

July 2006

Volume

120

Issue

1

Start / End Page

492 / 501

Location

United States

Related Subject Headings

  • Ultrasonography
  • Transcriptional Activation
  • Stress, Mechanical
  • Promoter Regions, Genetic
  • Neoplasms, Experimental
  • Mice, Inbred BALB C
  • Mice
  • Luminescent Agents
  • Luciferases
  • Hyperthermia, Induced