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Programmed cell death induced by ceramide.

Publication ,  Journal Article
Obeid, LM; Linardic, CM; Karolak, LA; Hannun, YA
Published in: Science
March 19, 1993

Sphingomyelin hydrolysis and ceramide generation have been implicated in a signal transduction pathway that mediates the effects of tumor necrosis factor-alpha (TNF-alpha) and other agents on cell growth and differentiation. In many leukemic cells, TNF-alpha causes DNA fragmentation, which leads to programmed cell death (apoptosis). C2-ceramide (0.6 to 5 microM), a synthetic cell-permeable ceramide analog, induced internucleosomal DNA fragmentation, which was inhibited by zinc ion. Other amphiphilic lipids failed to induce apoptosis. The closely related C2-dihydroceramide was also ineffective, which suggests a critical role for the sphingolipid double bond. The effects of C2-ceramide on DNA fragmentation were prevented by the protein kinase C activator phorbol 12-myristate 13-acetate, which suggests the existence of two opposing intracellular pathways in the regulation of apoptosis.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

March 19, 1993

Volume

259

Issue

5102

Start / End Page

1769 / 1771

Location

United States

Related Subject Headings

  • Zinc
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Sphingomyelins
  • Protein Kinase C
  • Nucleosomes
  • Leukemia
  • General Science & Technology
  • DNA Damage
 

Citation

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Obeid, L. M., Linardic, C. M., Karolak, L. A., & Hannun, Y. A. (1993). Programmed cell death induced by ceramide. Science, 259(5102), 1769–1771. https://doi.org/10.1126/science.8456305
Obeid, L. M., C. M. Linardic, L. A. Karolak, and Y. A. Hannun. “Programmed cell death induced by ceramide.Science 259, no. 5102 (March 19, 1993): 1769–71. https://doi.org/10.1126/science.8456305.
Obeid LM, Linardic CM, Karolak LA, Hannun YA. Programmed cell death induced by ceramide. Science. 1993 Mar 19;259(5102):1769–71.
Obeid, L. M., et al. “Programmed cell death induced by ceramide.Science, vol. 259, no. 5102, Mar. 1993, pp. 1769–71. Pubmed, doi:10.1126/science.8456305.
Obeid LM, Linardic CM, Karolak LA, Hannun YA. Programmed cell death induced by ceramide. Science. 1993 Mar 19;259(5102):1769–1771.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

March 19, 1993

Volume

259

Issue

5102

Start / End Page

1769 / 1771

Location

United States

Related Subject Headings

  • Zinc
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Tetradecanoylphorbol Acetate
  • Sphingomyelins
  • Protein Kinase C
  • Nucleosomes
  • Leukemia
  • General Science & Technology
  • DNA Damage