Degradation of proteoglycan in articular cartilage.
Journal Article (Journal Article)
Adult rabbit articular cartilage was labelled in vivo over 48 h with [35S]sulphate and was then incubated in organ culture at pH 7.2. Approx. 65% of the tissue content of [35S]proteoglycan was released into the culture medium during the first 48 h of incubation. The average molecular size of the released proteoglycans, as assessed by fractionation on Sepharose 2B/CL and 4B/Cl, was only slightly smaller than that of the proteoglycans extracted from non-cultured cartilage with 4 M guanidine HCl. The percentage of released proteoglycans and extracted proteoglycans which formed aggregates with hyaluronic acid was approx. 25% and 75%, respectively. The results indicate that proteoglycan degradation in adult articular cartilage is initiated by a limited proteolysis of subunit core protein, with the production of non-aggregating species which diffuse readily from the tissue.
Full Text
Duke Authors
Cited Authors
- Sandy, JD; Brown, HL; Lowther, DA
Published Date
- November 1, 1978
Published In
Volume / Issue
- 543 / 4
Start / End Page
- 536 - 544
PubMed ID
- 31190
International Standard Serial Number (ISSN)
- 0006-3002
Digital Object Identifier (DOI)
- 10.1016/0304-4165(78)90308-2
Language
- eng
Conference Location
- Netherlands