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rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease.

Publication ,  Journal Article
Byrne, FR; Farrell, CL; Aranda, R; Rex, KL; Scully, S; Brown, HL; Flores, SA; Gu, LH; Danilenko, DM; Lacey, DL; Ziegler, TR; Senaldi, G
Published in: Am J Physiol Gastrointest Liver Physiol
April 2002

There is an acute need for effective therapy for inflammatory bowel disease (IBD), particularly at the level of repair of the damaged epithelium. We evaluated the efficacy of recombinant human keratinocyte growth factor (rHuKGF) in both the dextran sodium sulfate (DSS) and the CD4(+)CD45RB(Hi) T cell transfer models of IBD. Disease was induced either by the ad libitum administration to normal mice of 4% DSS in the drinking water or by the injection of 4 x 10(5) CD4(+)CD45RB(Hi) T cells into immunodeficient scid/scid mice. rHuKGF was administered by subcutaneous injection at doses of 1.0 or 3.0 mg/kg in both preventative and therapeutic regimens during both studies. rHuKGF significantly improved survival and body weight loss in the DSS model in both preventative and therapeutic dosing regimens. It also improved diarrhea, hematochezia, and hematological parameters, as well as large intestine histopathology. In the T cell transfer model, rHuKGF improved body weight loss, diarrhea, and levels of serum amyloid A, as well as large intestine histopathology. In both models of IBD, the colonic levels of intestinal trefoil factor (ITF) were elevated by the disease state and further elevated by treatment with rHuKGF. These data suggest that rHuKGF may prove useful in the clinical management of IBD and its effects are likely mediated by its ability to locally increase the levels of ITF.

Duke Scholars

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

April 2002

Volume

282

Issue

4

Start / End Page

G690 / G701

Location

United States

Related Subject Headings

  • Weight Loss
  • Serum Amyloid A Protein
  • Recombinant Proteins
  • Neutrophils
  • Mice, SCID
  • Mice
  • Male
  • Leukocyte Count
  • Leukocyte Common Antigens
  • Intestinal Mucosa
 

Citation

APA
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Byrne, F. R., Farrell, C. L., Aranda, R., Rex, K. L., Scully, S., Brown, H. L., … Senaldi, G. (2002). rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol, 282(4), G690–G701. https://doi.org/10.1152/ajpgi.00314.2001
Byrne, Fergus R., Catherine L. Farrell, Richard Aranda, Karen L. Rex, Sheila Scully, Heather L. Brown, Silvia A. Flores, et al. “rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease.Am J Physiol Gastrointest Liver Physiol 282, no. 4 (April 2002): G690–701. https://doi.org/10.1152/ajpgi.00314.2001.
Byrne FR, Farrell CL, Aranda R, Rex KL, Scully S, Brown HL, et al. rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol. 2002 Apr;282(4):G690–701.
Byrne, Fergus R., et al. “rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease.Am J Physiol Gastrointest Liver Physiol, vol. 282, no. 4, Apr. 2002, pp. G690–701. Pubmed, doi:10.1152/ajpgi.00314.2001.
Byrne FR, Farrell CL, Aranda R, Rex KL, Scully S, Brown HL, Flores SA, Gu LH, Danilenko DM, Lacey DL, Ziegler TR, Senaldi G. rHuKGF ameliorates symptoms in DSS and CD4(+)CD45RB(Hi) T cell transfer mouse models of inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol. 2002 Apr;282(4):G690–G701.

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

April 2002

Volume

282

Issue

4

Start / End Page

G690 / G701

Location

United States

Related Subject Headings

  • Weight Loss
  • Serum Amyloid A Protein
  • Recombinant Proteins
  • Neutrophils
  • Mice, SCID
  • Mice
  • Male
  • Leukocyte Count
  • Leukocyte Common Antigens
  • Intestinal Mucosa