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Developmental aspects of sugar transport by isolated dog renal cortical tubules.

Publication ,  Journal Article
Foreman, JW; Medow, MS; Wald, H; Ginkinger, K; Segal, S
Published in: Pediatr Res
August 1984

alpha-Methyl-D-glucoside (AMG) uptake was examined in isolated renal cortical tubules from newborn, 3-month-old, and adult dogs. All three age groups demonstrated active sugar transport. The initial rate of AMG uptake was similar in the 3-month-old and adult tubules which was twice that of the newborn. At steady-state, the adult and newborn tubules had achieved a similar intracellular AMG concentration which was 45% greater than that of the 3-month-old. Determination of the flux constants of these uptake patterns revealed that there was an age-dependent increase in both the net flux and the fractional influx rate constant. However, the 3-month-old had the highest fractional efflux rate constant and the newborn the lowest value with the adult in between. Kinetic analysis of AMG uptake showed a single saturable transport system for each age group. The newborn and adult had similar Km values but the 3-month-old had a value that was 60% higher. The 3-month-old tubules had the highest Vmax and the newborn tubules the lowest with the adult value in between. AMG uptake by tubules from each age group demonstrated a similar pattern of inhibition in a low sodium buffer and by glucose and phlorizin. This indicated that, aside from kinetic changes with maturation, the saturable transport system for AMG is similar in each age group.

Duke Scholars

Published In

Pediatr Res

DOI

ISSN

0031-3998

Publication Date

August 1984

Volume

18

Issue

8

Start / End Page

719 / 723

Location

United States

Related Subject Headings

  • Water-Electrolyte Balance
  • Pediatrics
  • Methylglycosides
  • Methylglucosides
  • Male
  • Kinetics
  • Kidney Tubules, Proximal
  • Kidney Cortex
  • Female
  • Dogs
 

Citation

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Foreman, J. W., Medow, M. S., Wald, H., Ginkinger, K., & Segal, S. (1984). Developmental aspects of sugar transport by isolated dog renal cortical tubules. Pediatr Res, 18(8), 719–723. https://doi.org/10.1203/00006450-198408000-00008
Foreman, J. W., M. S. Medow, H. Wald, K. Ginkinger, and S. Segal. “Developmental aspects of sugar transport by isolated dog renal cortical tubules.Pediatr Res 18, no. 8 (August 1984): 719–23. https://doi.org/10.1203/00006450-198408000-00008.
Foreman JW, Medow MS, Wald H, Ginkinger K, Segal S. Developmental aspects of sugar transport by isolated dog renal cortical tubules. Pediatr Res. 1984 Aug;18(8):719–23.
Foreman, J. W., et al. “Developmental aspects of sugar transport by isolated dog renal cortical tubules.Pediatr Res, vol. 18, no. 8, Aug. 1984, pp. 719–23. Pubmed, doi:10.1203/00006450-198408000-00008.
Foreman JW, Medow MS, Wald H, Ginkinger K, Segal S. Developmental aspects of sugar transport by isolated dog renal cortical tubules. Pediatr Res. 1984 Aug;18(8):719–723.

Published In

Pediatr Res

DOI

ISSN

0031-3998

Publication Date

August 1984

Volume

18

Issue

8

Start / End Page

719 / 723

Location

United States

Related Subject Headings

  • Water-Electrolyte Balance
  • Pediatrics
  • Methylglycosides
  • Methylglucosides
  • Male
  • Kinetics
  • Kidney Tubules, Proximal
  • Kidney Cortex
  • Female
  • Dogs