Preservation of brush border transport systems for proline and alpha-methyl-D glucoside from rat, dog, and human kidney.

Published

Journal Article

The effects of freezing renal tissue from rat, dog, and man on the time course of uptake of proline and alpha-methyl-D-glucoside by subsequently isolated brush border membrane vesicles was examined and compared with uptake patterns by membranes isolated from tissue that had never been frozen. The overshoot phenomenon was used as the critical criterion for viability of the transport systems. Membranes isolated from frozen rat and dog kidney possessed intact transport systems for proline and alpha-methyl-D-glucoside capable of producing normal or even enhanced overshoot patterns. Freezing human kidney prior to membrane isolation resulted in severe impairment of the vesicle transport capabilities. Freezing a crude membrane suspension, however, allowed the subsequent purification of only partially intact systems.

Full Text

Duke Authors

Cited Authors

  • Pepe, LM; McNamara, PD; Foreman, JW; Tomassini, N; Hummeler, K; Segal, S

Published Date

  • December 1, 1982

Published In

Volume / Issue

  • 47 / 6

Start / End Page

  • 611 - 617

PubMed ID

  • 7144141

Pubmed Central ID

  • 7144141

International Standard Serial Number (ISSN)

  • 0023-6837

Language

  • eng

Conference Location

  • United States