Effect of cystine loading on substrate oxidation by rat renal tubules.

Published

Journal Article

Isolated rat renal tubules were loaded with cystine by incubating them with 2 mM cystine dimethylester. The oxidation of 1 mM glucose and lactate was significantly decreased after 20 and 30 min of incubation, in the cystine-loaded tubules compared with control tubules. The oxidation of 1 mM butyrate was significantly decreased after 10 and 30 min of incubation in the cystine-loaded tubules. Cystine loading decreased the oxidation of 1 mM succinate at all time points examined. The O2 consumption of renal tubules was reduced 59% with cystine loading by the addition of 2 mM cystine dimethylester, and by 37% with 1 mM cystine dimethylester. These data indicate that loading normal renal tubule cells with cystine impairs their ability to oxidize metabolic fuels. This impairment in metabolism may explain the decreased transport observed previously in cystine-loaded tubules and may have implications for the human disorder, cystinosis.

Full Text

Duke Authors

Cited Authors

  • Foreman, JW; Benson, LL

Published Date

  • May 1990

Published In

Volume / Issue

  • 4 / 3

Start / End Page

  • 236 - 239

PubMed ID

  • 2119211

Pubmed Central ID

  • 2119211

International Standard Serial Number (ISSN)

  • 0931-041X

Digital Object Identifier (DOI)

  • 10.1007/bf00857662

Language

  • eng

Conference Location

  • Germany