Effect of cystine loading and cystine dimethylester on renal brushborder membrane transport.

Journal Article

The effect of loading renal tubule cells with cystine was studied by incubating them with cystine dimethylester. Proline uptake into brushborder membrane vesicles isolated from the cystine loaded cells was not different from that observed into brushborder vesicles isolated from tubules incubated in buffer alone. Incubating brushborder membranes with 2 mM cystine dimethylester for 10 minutes reduced the uptake of proline by 27% after 15 seconds of incubation and by 21% after 60 seconds of incubation. There was no effect after 20 minutes of incubation. Pre-incubating brushborder membrane vesicles with cystine dimethylester had no statistically significant effect on the affinity of proline for the carrier, but did reduce the maximal rate of proline uptake by 49%.

Full Text

Duke Authors

Cited Authors

  • Foreman, JW; Benson, L

Published Date

  • October 1990

Published In

Volume / Issue

  • 10 / 5

Start / End Page

  • 455 - 459

PubMed ID

  • 2282372

Pubmed Central ID

  • 2282372

International Standard Serial Number (ISSN)

  • 0144-8463

Digital Object Identifier (DOI)

  • 10.1007/BF01152292

Language

  • eng

Conference Location

  • England