Effect of acidosis on glutamine transport by isolated rat renal brush-border and basolateral-membrane vesicles.

Published

Journal Article

Glutamine uptake was examined in isolated renal brush-border and basolateral-membrane vesicles from control and acidotic rats. In brush-border vesicles from acidotic animals, there was a significant increase in the initial rate of glutamine uptake compared with that in controls. Lowering the pH of the medium increased the initial rate of glutamine uptake in brush-border vesicles from acidotic, but not from control, rats. In brush-border vesicles from both groups of animals, two saturable transport systems mediated glutamine uptake. There was a 2-fold increase in the Vmax. of the low-affinity high-capacity system in the brush-border vesicles from the acidotic animals compared with that from control animals, with no alteration in the other kinetic parameters. There was no difference in glutamine uptake by the two saturable transport systems in basolateral vesicles from control and acidotic animals. Lowering the incubation-medium pH increased the uptake of glutamine by basolateral vesicles from both control and acidotic rats to a similar extent. The data indicate that during acidosis there are alterations in glutamine transport by both the basolateral and brush-border membrane which could enhance its uptake by the renal-tubule cell for use in ammoniagenesis.

Full Text

Duke Authors

Cited Authors

  • Foreman, JW; Reynolds, RA; Ginkinger, K; Segal, S

Published Date

  • June 15, 1983

Published In

Volume / Issue

  • 212 / 3

Start / End Page

  • 713 - 720

PubMed ID

  • 6882392

Pubmed Central ID

  • 6882392

International Standard Serial Number (ISSN)

  • 0264-6021

Digital Object Identifier (DOI)

  • 10.1042/bj2120713

Language

  • eng

Conference Location

  • England