Characteristics of lysine transport by isolated rat renal cortical tubule fragments.
Journal Article
The uptake of L-lysine was examined in isolated renal cortical tubules. Lysine was actively taken up by the renal tubule cells isolated from 7-week-old rats. No metabolism of the transported lysine was found. There was no evidence for sodium-dependence of lysine uptake. Concentration dependence studies revealed that the lysine was taken up by one saturable transport system with a Km of 1.66 mmol/l and Vmax of 7 mmol/l intracellular fluid per 10 min. Lysine also entered by a non-saturable pathway. Arginine and ornithine inhibited the initial uptake of lysine. Cystine increased the efflux of lysine from preloaded renal cells via hetero-exchange, indicating that a common system exists for these two amino acids.
Full Text
Duke Authors
Cited Authors
- Bowring, MA; Foreman, JW; Lee, J; Segal, S
Published Date
- July 10, 1987
Published In
Volume / Issue
- 901 / 1
Start / End Page
- 23 - 29
PubMed ID
- 3109479
Pubmed Central ID
- 3109479
International Standard Serial Number (ISSN)
- 0006-3002
Digital Object Identifier (DOI)
- 10.1016/0005-2736(87)90252-5
Language
- eng
Conference Location
- Netherlands