Characteristics of lysine transport by isolated rat renal cortical tubule fragments.

Journal Article (Journal Article)

The uptake of L-lysine was examined in isolated renal cortical tubules. Lysine was actively taken up by the renal tubule cells isolated from 7-week-old rats. No metabolism of the transported lysine was found. There was no evidence for sodium-dependence of lysine uptake. Concentration dependence studies revealed that the lysine was taken up by one saturable transport system with a Km of 1.66 mmol/l and Vmax of 7 mmol/l intracellular fluid per 10 min. Lysine also entered by a non-saturable pathway. Arginine and ornithine inhibited the initial uptake of lysine. Cystine increased the efflux of lysine from preloaded renal cells via hetero-exchange, indicating that a common system exists for these two amino acids.

Full Text

Duke Authors

Cited Authors

  • Bowring, MA; Foreman, JW; Lee, J; Segal, S

Published Date

  • July 10, 1987

Published In

Volume / Issue

  • 901 / 1

Start / End Page

  • 23 - 29

PubMed ID

  • 3109479

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0005-2736(87)90252-5


  • eng

Conference Location

  • Netherlands