Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.

Journal Article (Journal Article)

The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium oxalate, most humans do not form stones. Inhibitors are among the multiple factors that may influence the complex process of urinary stone formation. We have isolated an inhibitor of calcium oxalate crystal growth from human urine by monoclonal antibody immunoaffinity chromatography. The N-terminal amino acid sequence and acidic amino acid content of this aspartic acid-rich protein, uropontin, are similar to those of other pontin proteins from bone, plasma, breast milk, and cells. The inhibitory effect of uropontin on calcium oxalate crystal growth in vitro supports the concept that pontins may have a regulatory role. This function would be analogous to that of other members of the aspartic acid-rich protein superfamily, which stereospecifically regulate the mineralization fronts of calcium-containing crystals.

Full Text

Duke Authors

Cited Authors

  • Shiraga, H; Min, W; VanDusen, WJ; Clayman, MD; Miner, D; Terrell, CH; Sherbotie, JR; Foreman, JW; Przysiecki, C; Neilson, EG

Published Date

  • January 1, 1992

Published In

Volume / Issue

  • 89 / 1

Start / End Page

  • 426 - 430

PubMed ID

  • 1729712

Pubmed Central ID

  • PMC48250

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.89.1.426


  • eng

Conference Location

  • United States