Chemotherapy-induced apoptosis in epithelial ovarian cancers.

Journal Article

Objective

To determine whether chemotherapy drugs elicit programmed cell death (apoptosis) in ovarian cancer cells.

Methods

Monolayers of immortalized ovarian cancer cell lines and primary ovarian cancer cells obtained from ascites were grown in the presence of cisplatin, 4-hydroxyperoxy-cyclophosphamide (the active metabolite of cyclophosphamide) or paclitaxel. Next, DNA was extracted from the cells and subjected to electrophoresis to determine if DNA laddering characteristic of apoptosis was present.

Results

In three of six immortalized cell lines (OVCA 420, 429, and 433), apoptosis was not seen in response to any of the three drugs. In contrast, in OVCAR-3 and OVCA 432, DNA laddering consistent with apoptosis was observed in response to all three drugs. In the DOV 13 cell line, apoptosis was seen only with 4-hydroxyperoxycyclophosphamide. Among three primary ovarian cancers, cisplatin elicited apoptosis in one case. Both cell lines with mutant p53 genes (OVCAR-3 and OVCA 432) underwent apoptosis in response to all three drugs, whereas among three cell lines known to have normal p53 genes, one underwent apoptosis in response to 4-hydroxyperoxycyclophosphamide and two were unaffected.

Conclusion

Ovarian cancer cell death in response to commonly used chemotherapeutic drugs involves the induction of a genetically programmed sequence of events (apoptosis) rather than simply necrosis.

Full Text

Duke Authors

Cited Authors

  • Havrilesky, LJ; Elbendary, A; Hurteau, JA; Whitaker, RS; Rodriguez, GC; Berchuck, A

Published Date

  • June 1995

Published In

Volume / Issue

  • 85 / 6

Start / End Page

  • 1007 - 1010

PubMed ID

  • 7770245

Pubmed Central ID

  • 7770245

Electronic International Standard Serial Number (EISSN)

  • 1873-233X

International Standard Serial Number (ISSN)

  • 0029-7844

Digital Object Identifier (DOI)

  • 10.1016/0029-7844(95)00058-y

Language

  • eng