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Chemotherapy-induced apoptosis in epithelial ovarian cancers.

Publication ,  Journal Article
Havrilesky, LJ; Elbendary, A; Hurteau, JA; Whitaker, RS; Rodriguez, GC; Berchuck, A
Published in: Obstet Gynecol
June 1995

OBJECTIVE: To determine whether chemotherapy drugs elicit programmed cell death (apoptosis) in ovarian cancer cells. METHODS: Monolayers of immortalized ovarian cancer cell lines and primary ovarian cancer cells obtained from ascites were grown in the presence of cisplatin, 4-hydroxyperoxy-cyclophosphamide (the active metabolite of cyclophosphamide) or paclitaxel. Next, DNA was extracted from the cells and subjected to electrophoresis to determine if DNA laddering characteristic of apoptosis was present. RESULTS: In three of six immortalized cell lines (OVCA 420, 429, and 433), apoptosis was not seen in response to any of the three drugs. In contrast, in OVCAR-3 and OVCA 432, DNA laddering consistent with apoptosis was observed in response to all three drugs. In the DOV 13 cell line, apoptosis was seen only with 4-hydroxyperoxycyclophosphamide. Among three primary ovarian cancers, cisplatin elicited apoptosis in one case. Both cell lines with mutant p53 genes (OVCAR-3 and OVCA 432) underwent apoptosis in response to all three drugs, whereas among three cell lines known to have normal p53 genes, one underwent apoptosis in response to 4-hydroxyperoxycyclophosphamide and two were unaffected. CONCLUSION: Ovarian cancer cell death in response to commonly used chemotherapeutic drugs involves the induction of a genetically programmed sequence of events (apoptosis) rather than simply necrosis.

Duke Scholars

Published In

Obstet Gynecol

DOI

ISSN

0029-7844

Publication Date

June 1995

Volume

85

Issue

6

Start / End Page

1007 / 1010

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Paclitaxel
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female
  • DNA, Neoplasm
  • Cyclophosphamide
  • Cisplatin
  • Apoptosis
 

Citation

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Havrilesky, L. J., Elbendary, A., Hurteau, J. A., Whitaker, R. S., Rodriguez, G. C., & Berchuck, A. (1995). Chemotherapy-induced apoptosis in epithelial ovarian cancers. Obstet Gynecol, 85(6), 1007–1010. https://doi.org/10.1016/0029-7844(95)00058-y
Havrilesky, L. J., A. Elbendary, J. A. Hurteau, R. S. Whitaker, G. C. Rodriguez, and A. Berchuck. “Chemotherapy-induced apoptosis in epithelial ovarian cancers.Obstet Gynecol 85, no. 6 (June 1995): 1007–10. https://doi.org/10.1016/0029-7844(95)00058-y.
Havrilesky LJ, Elbendary A, Hurteau JA, Whitaker RS, Rodriguez GC, Berchuck A. Chemotherapy-induced apoptosis in epithelial ovarian cancers. Obstet Gynecol. 1995 Jun;85(6):1007–10.
Havrilesky, L. J., et al. “Chemotherapy-induced apoptosis in epithelial ovarian cancers.Obstet Gynecol, vol. 85, no. 6, June 1995, pp. 1007–10. Pubmed, doi:10.1016/0029-7844(95)00058-y.
Havrilesky LJ, Elbendary A, Hurteau JA, Whitaker RS, Rodriguez GC, Berchuck A. Chemotherapy-induced apoptosis in epithelial ovarian cancers. Obstet Gynecol. 1995 Jun;85(6):1007–1010.
Journal cover image

Published In

Obstet Gynecol

DOI

ISSN

0029-7844

Publication Date

June 1995

Volume

85

Issue

6

Start / End Page

1007 / 1010

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Paclitaxel
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Humans
  • Female
  • DNA, Neoplasm
  • Cyclophosphamide
  • Cisplatin
  • Apoptosis