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MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.

Publication ,  Journal Article
Li, Q-J; Yang, S-H; Maeda, Y; Sladek, FM; Sharrocks, AD; Martins-Green, M
Published in: EMBO J
January 15, 2003

CBP/p300 recruitment to enhancer-bound complexes is a key determinant in promoter activation by many transcription factors. We present a novel mechanism of activating such complexes and show that pre-assembled Elk-1-p300 complexes become activated following Elk-1 phosphorylation by changes in Elk-1-p300 interactions rather than recruitment. It is known that Elk-1 binds to promoter in the absence of stimuli. However, it is unclear how activation of Elk-1 by mitogen-acivated protein kinase (MAPK)-mediated phosphorylation leads to targeted gene transactivation. We show that Elk-1 can interact with p300 in vitro and in vivo in the absence of a stimulus through the Elk-1 C-terminus and the p300 N-terminus. Phosphorylation on Ser383 and Ser389 of Elk-1 by MAPK enhances this basal binding but, most importantly, Elk-1 exhibits new interactions with p300. These interaction changes render a strong histone acetyltransferase activity in the Elk-1-associated complex that could play a critical role in chromatin remodeling and gene activation. The pre-assembly mechanism may greatly accelerate transcription activation, which is important in regulation of expression of immediate-early response genes, in particular those involved in stress responses.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

January 15, 2003

Volume

22

Issue

2

Start / End Page

281 / 291

Location

England

Related Subject Headings

  • ets-Domain Protein Elk-1
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Thrombin
  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Phosphorylation
 

Citation

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Li, Q.-J., Yang, S.-H., Maeda, Y., Sladek, F. M., Sharrocks, A. D., & Martins-Green, M. (2003). MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J, 22(2), 281–291. https://doi.org/10.1093/emboj/cdg028
Li, Qi-Jing, Shen-Hsi Yang, Yutaka Maeda, Frances M. Sladek, Andrew D. Sharrocks, and Manuela Martins-Green. “MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.EMBO J 22, no. 2 (January 15, 2003): 281–91. https://doi.org/10.1093/emboj/cdg028.
Li Q-J, Yang S-H, Maeda Y, Sladek FM, Sharrocks AD, Martins-Green M. MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J. 2003 Jan 15;22(2):281–91.
Li, Qi-Jing, et al. “MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.EMBO J, vol. 22, no. 2, Jan. 2003, pp. 281–91. Pubmed, doi:10.1093/emboj/cdg028.
Li Q-J, Yang S-H, Maeda Y, Sladek FM, Sharrocks AD, Martins-Green M. MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J. 2003 Jan 15;22(2):281–291.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

January 15, 2003

Volume

22

Issue

2

Start / End Page

281 / 291

Location

England

Related Subject Headings

  • ets-Domain Protein Elk-1
  • Transcriptional Activation
  • Transcription Factors
  • Trans-Activators
  • Thrombin
  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Promoter Regions, Genetic
  • Phosphorylation