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A new generation organ culture arising from cross-talk between multiple primary human cell types.

Publication ,  Journal Article
Martins-Green, M; Li, Q-J; Yao, M
Published in: FASEB J
February 2005

The inability to experiment directly on humans strongly constrains biomedical research, creating a great need to develop cultures that mimic human tissues and organs as experimental systems that can be used to directly understand and manipulate biological processes. The advent of availability of primary human cells now makes possible engineering of such organ cultures. Here we report the generation of a human "skin" arguably the simplest human tissue. Beginning with three primary cell types taken from adult tissues, this organ culture develops into a mature tissue containing a stratified epithelium and an interconnected network of mature microvessels, with appropriate matrix molecules and cytokines. Surprisingly, pericytes and monocytes appear adjacent to and within "blood" vessels, respectively. These cultures respond appropriately to stimulators of specific biological processes, providing a vehicle to investigate basic biological processes, such as 1) cell-cell and cell-microenvironment interaction; 2) transdifferentiation of one cell type to another and/or differentiation from stem cells present in adult tissues; and 3) opportunities for genetic manipulation of human tissues to understand function. Moreover, this "skin" can potentially be developed into a tailored "living bandage" for patients with impaired healing and can serve as prototype for the development of other human organ cultures.

Duke Scholars

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

February 2005

Volume

19

Issue

2

Start / End Page

222 / 224

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Skin
  • Organ Culture Techniques
  • Keratinocytes
  • Humans
  • Fibroblasts
  • Fibrillar Collagens
  • Extracellular Matrix
  • Endothelium, Vascular
  • Endothelial Cells
 

Citation

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ICMJE
MLA
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Martins-Green, M., Li, Q.-J., & Yao, M. (2005). A new generation organ culture arising from cross-talk between multiple primary human cell types. FASEB J, 19(2), 222–224. https://doi.org/10.1096/fj.04-1725fje
Martins-Green, Manuela, Qi-Jing Li, and Min Yao. “A new generation organ culture arising from cross-talk between multiple primary human cell types.FASEB J 19, no. 2 (February 2005): 222–24. https://doi.org/10.1096/fj.04-1725fje.
Martins-Green M, Li Q-J, Yao M. A new generation organ culture arising from cross-talk between multiple primary human cell types. FASEB J. 2005 Feb;19(2):222–4.
Martins-Green, Manuela, et al. “A new generation organ culture arising from cross-talk between multiple primary human cell types.FASEB J, vol. 19, no. 2, Feb. 2005, pp. 222–24. Pubmed, doi:10.1096/fj.04-1725fje.
Martins-Green M, Li Q-J, Yao M. A new generation organ culture arising from cross-talk between multiple primary human cell types. FASEB J. 2005 Feb;19(2):222–224.

Published In

FASEB J

DOI

EISSN

1530-6860

Publication Date

February 2005

Volume

19

Issue

2

Start / End Page

222 / 224

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Skin
  • Organ Culture Techniques
  • Keratinocytes
  • Humans
  • Fibroblasts
  • Fibrillar Collagens
  • Extracellular Matrix
  • Endothelium, Vascular
  • Endothelial Cells