Histochemical localization of the PBAN receptor in the pheromone gland of Heliothis peltigera.

Published

Journal Article

The presence of the pyrokinin (PK)/ Pheromone biosynthesis activating neuropeptide (PBAN) receptor in pheromone gland cells of Heliothis peltigera females was demonstrated, and its spatial distribution in the ovipositor was visualized with two photo-affinity biotinilated ligands: BpaPBAN1-33NH(2) and BpaArg(27)-PBAN28-33NH(2). Light microscopy histological studies revealed that the gland is contained within the inter-segmental membrane (ISM) between the 8th and 9th abdominal segments. The gland was found to be composed of a single layer of columnar epithelial cells positioned under the inter-segmental cuticle. Similar epithelial cells were also found in the dorsal and ventral regions of the 9th abdominal segment. All regions containing the glandular cells bound both ligands, indicating presence of the PK/PBAN receptor. The patterns obtained with both ligands were similar, hinting at the possibility that either both ligands bind to the same receptor, or, that if there are two distinct receptors, their spatial distribution throughout the gland is very similar.

Full Text

Duke Authors

Cited Authors

  • Altstein, M; Ben-Aziz, O; Bhargava, K; Li, Q; Martins-Green, M

Published Date

  • September 2003

Published In

Volume / Issue

  • 24 / 9

Start / End Page

  • 1335 - 1347

PubMed ID

  • 14706548

Pubmed Central ID

  • 14706548

International Standard Serial Number (ISSN)

  • 0196-9781

Language

  • eng

Conference Location

  • United States