A retrovirus carrying the promyelocyte-retinoic acid receptor PML-RARalpha fusion gene transforms haematopoietic progenitors in vitro and induces acute leukaemias.

Journal Article

The promyelocyte (PML)-retinoic acid receptor alpha (RARalpha) fusion gene results from a t(15;17) chromosome translocation in acute promyelocytic leukaemia. We have analysed the oncogenic potential of the human fusion PML-RARalpha product in chicken using retrovirus vectors. We show that PML-RARalpha transforms very early haematopoietic progenitor cells in vitro and induces acute leukaemias. Neither PML nor RARalpha domains alone achieve such a transformation. The PML-RARalpha viruses recovered from the transformed cells carry two point mutations in the PML domain, one of which alters both the pattern of intracellular localization of the fusion protein and its functional interference with AP-1, thus defining an essential domain in PML for oncogenic transformation.

Full Text

Duke Authors

Cited Authors

  • Altabef, M; Garcia, M; Lavau, C; Bae, SC; Dejean, A; Samarut, J

Published Date

  • June 3, 1996

Published In

Volume / Issue

  • 15 / 11

Start / End Page

  • 2707 - 2716

PubMed ID

  • 8654368

International Standard Serial Number (ISSN)

  • 0261-4189

Language

  • eng

Conference Location

  • England