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Prolactin receptor gene expression in the fetal rat.

Publication ,  Journal Article
Freemark, M; Nagano, M; Edery, M; Kelly, PA
Published in: J Endocrinol
February 1995

The expression of mRNA encoding the long and short forms of the prolactin receptor (PRLR) in the fetal rat was examined using the method of reverse transcription-PCR. A 742 bp PCR product encoding the extracellular and transmembrane domains of the PRLR was detected in maternal and fetal liver and in fetal adrenal, kidney, small intestine, pancreas, brain, pituitary, thymus, lung and skin but not in fetal heart. Highest levels of the 742 bp PRLR transcript were detected in fetal adrenal (45.2% of levels in maternal liver), kidney (27.2%), small intestine (21.7%), pancreas (18.3%) and liver (10.8%), and tissue levels of the 742 bp product correlated positively (r = 0.92, P < 0.01) with the specific binding of the fetal lactogenic hormone rat placental lactogen II (rPL-II). These findings suggest that the PRLR may serve as a physiological binding protein for rPL-II in the rat fetus. There were striking differences in the relative expression of mRNA encoding the long and short forms of the PRLR. The long form of the receptor was expressed in maternal liver and placenta and in all fetal tissues studied except fetal heart. The short form of the receptor was also detected in maternal liver and placenta and fetal adrenal, kidney, small intestine, liver and thymus; in contrast, there was limited expression of the short-form of the receptor in fetal pancreas, pituitary and brain and no short form transcripts were detected in fetal lung, skin or heart.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

J Endocrinol

DOI

ISSN

0022-0795

Publication Date

February 1995

Volume

144

Issue

2

Start / End Page

285 / 292

Location

England

Related Subject Headings

  • Receptors, Prolactin
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Binding
  • Prolactin
  • Polymerase Chain Reaction
  • Placenta
  • Pancreas
  • Molecular Sequence Data
 

Citation

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Freemark, M., Nagano, M., Edery, M., & Kelly, P. A. (1995). Prolactin receptor gene expression in the fetal rat. J Endocrinol, 144(2), 285–292. https://doi.org/10.1677/joe.0.1440285
Freemark, M., M. Nagano, M. Edery, and P. A. Kelly. “Prolactin receptor gene expression in the fetal rat.J Endocrinol 144, no. 2 (February 1995): 285–92. https://doi.org/10.1677/joe.0.1440285.
Freemark M, Nagano M, Edery M, Kelly PA. Prolactin receptor gene expression in the fetal rat. J Endocrinol. 1995 Feb;144(2):285–92.
Freemark, M., et al. “Prolactin receptor gene expression in the fetal rat.J Endocrinol, vol. 144, no. 2, Feb. 1995, pp. 285–92. Pubmed, doi:10.1677/joe.0.1440285.
Freemark M, Nagano M, Edery M, Kelly PA. Prolactin receptor gene expression in the fetal rat. J Endocrinol. 1995 Feb;144(2):285–292.
Journal cover image

Published In

J Endocrinol

DOI

ISSN

0022-0795

Publication Date

February 1995

Volume

144

Issue

2

Start / End Page

285 / 292

Location

England

Related Subject Headings

  • Receptors, Prolactin
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Binding
  • Prolactin
  • Polymerase Chain Reaction
  • Placenta
  • Pancreas
  • Molecular Sequence Data