A microliter oxygen electrode system for sperm suspensions.
Publication
, Journal Article
Katz, DF; Blake, ET; Mills, RN; Fatt, I
Published in: Fertility and sterility
December 1978
A new polarographic oxygen electrode system is described which can accept sperm suspensions with volumes as low as 50 microliters. In this configuration, the electrode tip forms the base of the suspension reaction chamber, and there is no stirring. Consequently, structured fluids such as cervical mucus can be applied. Measurements can be performed in as little time as 10 minutes. The system reliably measures oxygen consumption rates as low as 0.1 mm Hg/minute, so that mammalian sperm suspensions with concentrations of the order 1 X 10(6)viable cells/ml can be applied. Sample experiments, comparing the oxygen uptake of human spermatozoa in semen and in Tyrode's solution, are described.
Duke Scholars
Published In
Fertility and sterility
DOI
EISSN
1556-5653
ISSN
0015-0282
Publication Date
December 1978
Volume
30
Issue
6
Start / End Page
691 / 695
Related Subject Headings
- Spermatozoa
- Polarography
- Oxygen Consumption
- Obstetrics & Reproductive Medicine
- Male
- Humans
- Electrodes
- Adult
- 3215 Reproductive medicine
- 1117 Public Health and Health Services
Citation
APA
Chicago
ICMJE
MLA
NLM
Katz, D. F., Blake, E. T., Mills, R. N., & Fatt, I. (1978). A microliter oxygen electrode system for sperm suspensions. Fertility and Sterility, 30(6), 691–695. https://doi.org/10.1016/s0015-0282(16)43698-8
Katz, D. F., E. T. Blake, R. N. Mills, and I. Fatt. “A microliter oxygen electrode system for sperm suspensions.” Fertility and Sterility 30, no. 6 (December 1978): 691–95. https://doi.org/10.1016/s0015-0282(16)43698-8.
Katz DF, Blake ET, Mills RN, Fatt I. A microliter oxygen electrode system for sperm suspensions. Fertility and sterility. 1978 Dec;30(6):691–5.
Katz, D. F., et al. “A microliter oxygen electrode system for sperm suspensions.” Fertility and Sterility, vol. 30, no. 6, Dec. 1978, pp. 691–95. Epmc, doi:10.1016/s0015-0282(16)43698-8.
Katz DF, Blake ET, Mills RN, Fatt I. A microliter oxygen electrode system for sperm suspensions. Fertility and sterility. 1978 Dec;30(6):691–695.
Published In
Fertility and sterility
DOI
EISSN
1556-5653
ISSN
0015-0282
Publication Date
December 1978
Volume
30
Issue
6
Start / End Page
691 / 695
Related Subject Headings
- Spermatozoa
- Polarography
- Oxygen Consumption
- Obstetrics & Reproductive Medicine
- Male
- Humans
- Electrodes
- Adult
- 3215 Reproductive medicine
- 1117 Public Health and Health Services