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Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death.

Publication ,  Journal Article
Dbaibo, GS; El-Assaad, W; Krikorian, A; Liu, B; Diab, K; Idriss, NZ; El-Sabban, M; Driscoll, TA; Perry, DK; Hannun, YA
Published in: FEBS Lett
August 10, 2001

Ceramide accumulation in the cell can occur from either hydrolysis of sphingomyelin or by de novo synthesis. In this study, we found that blocking de novo ceramide synthesis significantly inhibits ceramide accumulation and subsequent cell death in response to tumor necrosis factor alpha. When cells were pre-treated with glutathione, a proposed cellular regulator of neutral sphingomyelinase, inhibition of ceramide accumulation at early time points was achieved with attenuation of cell death. Inhibition of both pathways achieved near-complete inhibition of ceramide accumulation and cell death indicating that both pathways of ceramide generation are stimulated. This illustrates the complexity of ceramide generation in cytokine action.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

August 10, 2001

Volume

503

Issue

1

Start / End Page

7 / 12

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Humans
  • Glutathione
  • Ceramides
  • Biochemistry & Molecular Biology
  • Apoptosis
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology
  • 0601 Biochemistry and Cell Biology
 

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Dbaibo, G. S., El-Assaad, W., Krikorian, A., Liu, B., Diab, K., Idriss, N. Z., … Hannun, Y. A. (2001). Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death. FEBS Lett, 503(1), 7–12. https://doi.org/10.1016/s0014-5793(01)02625-4
Dbaibo, G. S., W. El-Assaad, A. Krikorian, B. Liu, K. Diab, N. Z. Idriss, M. El-Sabban, T. A. Driscoll, D. K. Perry, and Y. A. Hannun. “Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death.FEBS Lett 503, no. 1 (August 10, 2001): 7–12. https://doi.org/10.1016/s0014-5793(01)02625-4.
Dbaibo GS, El-Assaad W, Krikorian A, Liu B, Diab K, Idriss NZ, et al. Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death. FEBS Lett. 2001 Aug 10;503(1):7–12.
Dbaibo, G. S., et al. “Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death.FEBS Lett, vol. 503, no. 1, Aug. 2001, pp. 7–12. Pubmed, doi:10.1016/s0014-5793(01)02625-4.
Dbaibo GS, El-Assaad W, Krikorian A, Liu B, Diab K, Idriss NZ, El-Sabban M, Driscoll TA, Perry DK, Hannun YA. Ceramide generation by two distinct pathways in tumor necrosis factor alpha-induced cell death. FEBS Lett. 2001 Aug 10;503(1):7–12.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

August 10, 2001

Volume

503

Issue

1

Start / End Page

7 / 12

Location

England

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Humans
  • Glutathione
  • Ceramides
  • Biochemistry & Molecular Biology
  • Apoptosis
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology
  • 0601 Biochemistry and Cell Biology