A one-step large-scale method for T- and B-cell depletion of mobilized PBSC for allogeneic transplantation.

Published

Journal Article

BACKGROUND: The presence of T and B cells in allogeneic grafts contributes to GvHD and to EBV-associated lymphoproliferative disease (LPD). Depletion of T and B cells from the graft decreases the risk of these complications. METHODS: T and B cells were depleted from mobilized peripheral stem cells from volunteer donors (n=5) using anti-CD3 and anti-CD19 Abs conjugated to magnetic microbeads, and the CliniMACS device. The function of the stem cells after depletion was evaluated using colony assays and non-obese diabetic (NOD)/SCID repopulating experiments. RESULTS: The mean mononuclear cell (MNC) count prior to T- and B-cell depletion was 2.19x10(10) (range 1.48-3.53). After depletion, the mean percentage of contaminating T cells was 0.02% (range 0.01-0.04%) with a mean log(10) depletion of 3.4 (range 3-3.8). The mean percentage of contaminating B cells was 0.1% (range 0.01-0.4%) with a mean log(10) depletion of 2.2 (range 1.4-3). The mean recovery of CD3- and CD19-negative MNCs after depletion was 70% (range 54-88%) and the mean recovery of CD34(+) stem cells was 69% (range 52-98%). The mean number of natural killer (NK) cells after T- and B-cell depletion was 5.2x10(8) (range 2-10x10(8)). In vitro colony assays and in vivo NOD/SCID repopulation assays showed no negative impact of this method on the function of the hematopoietic stem cells. DISCUSSION: Our results show that the CliniMACS system can be used to efficiently deplete PBSC of T and B cells simultaneously, without adverse effect on the graft.

Full Text

Duke Authors

Cited Authors

  • Barfield, RC; Otto, M; Houston, J; Holladay, M; Geiger, T; Martin, J; Leimig, T; Gordon, P; Chen, X; Handgretinger, R

Published Date

  • 2004

Published In

Volume / Issue

  • 6 / 1

Start / End Page

  • 1 - 6

PubMed ID

  • 14985161

Pubmed Central ID

  • 14985161

International Standard Serial Number (ISSN)

  • 1465-3249

Digital Object Identifier (DOI)

  • 10.1080/14653240310004411

Language

  • eng

Conference Location

  • England