A large-scale method for the selective depletion of alphabeta T lymphocytes from PBSC for allogeneic transplantation.

Journal Article

BACKGROUND: We sought to develop a method for the clinical large-scale depletion of alphabeta T lymphocytes from mobilized peripheral stem cells, which would allow the allogeneic transplantation of a graft enriched for stem cells, natural killer (NK) cells and gammadelta T lymphocytes. METHODS: Therefore, we obtained mononuclear cells from either mobilized or non-mobilized healthy adult volunteer donors and incubated the cells with a biotinylated anti-alphabeta T-cell Ab and subsequently with an anti-biotin Ab conjugated with magnetic microbeads. The depletion was then performed using a CliniMACS device. RESULTS: The median T-cell depletion was 3.9 log (range 3.5-4.1 log). The recovery of the gammadelta and NK cells was 92% and 80%, respectively. The recovery of CD34+ stem cells from the mobilized donors was 66%. DISCUSSION: This method had no negative influence on the in vitro colony formation of stem cells, and transplantation of alphabeta-depleted cells into NOD-SCID IL-2 common gamma chain knockout (NOD-scid IL2r (null)) mice resulted in a rapid engraftment of human myeloid and lymphoid cells. This method will allow large-scale depletion of alphabeta T cells from mobilized peripheral blood in clinical trials.

Full Text

Duke Authors

Cited Authors

  • Chaleff, S; Otto, M; Barfield, RC; Leimig, T; Iyengar, R; Martin, J; Holiday, M; Houston, J; Geiger, T; Huppert, V; Handgretinger, R

Published Date

  • 2007

Published In

Volume / Issue

  • 9 / 8

Start / End Page

  • 746 - 754

PubMed ID

  • 17917892

Electronic International Standard Serial Number (EISSN)

  • 1477-2566

Digital Object Identifier (DOI)

  • 10.1080/14653240701644000

Language

  • eng

Conference Location

  • England