Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.
Published
Journal Article (Review)
Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm(3). A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.
Full Text
Duke Authors
Cited Authors
- Oldham, M; Sakhalkar, H; Oliver, T; Allan Johnson, G; Dewhirst, M
Published Date
- March 2008
Published In
Volume / Issue
- 13 / 2
Start / End Page
- 021113 -
PubMed ID
- 18465962
Pubmed Central ID
- 18465962
International Standard Serial Number (ISSN)
- 1083-3668
Digital Object Identifier (DOI)
- 10.1117/1.2907968
Language
- eng
Conference Location
- United States