Optical clearing of unsectioned specimens for three-dimensional imaging via optical transmission and emission tomography.


Journal Article (Review)

Optical computed tomography (optical-CT) and optical emission computed tomography (optical-ECT) are new techniques that enable unprecedented high-resolution 3-D multimodal imaging of tissue structure and function. Applications include imaging macroscopic gene expression and microvasculature structure in unsectioned biological specimens up to 8 cm(3). A key requisite for these imaging techniques is effective sample preparation including optical clearing, which enables light transport through the sample while preserving the signal (either light absorbing stain or fluorescent proteins) in representative form. We review recent developments in optical-CT and optical-ECT, and compatible "fluorescence-friendly" optical clearing protocols.

Full Text

Duke Authors

Cited Authors

  • Oldham, M; Sakhalkar, H; Oliver, T; Allan Johnson, G; Dewhirst, M

Published Date

  • March 2008

Published In

Volume / Issue

  • 13 / 2

Start / End Page

  • 021113 -

PubMed ID

  • 18465962

Pubmed Central ID

  • 18465962

International Standard Serial Number (ISSN)

  • 1083-3668

Digital Object Identifier (DOI)

  • 10.1117/1.2907968


  • eng

Conference Location

  • United States