Effect of surrogate tolerogenesis on the vascular rejection of pig heart xenografts.

Journal Article (Journal Article)

BACKGROUND: Organ xenografts are fulminantly rejected by antibody-mediated vascular rejection. Surrogate tolerogenesis (ST), the induction of tolerance within the donor, is effective with aorta xenografts. This preliminary study assesses the effect of ST on preformed antibodies and rejection of porcine heart xenografts. METHODS: Tolerance to the donor pig was induced by infusing recipient marrow into fetal pigs. Later, pig splenocytes were transfused and heterotopic pig hearts transplanted using chimeric or nonchimeric pigs. Anti-pig antibodies were assessed. RESULTS: With ST alone, xenografts developed cellular rejection at 4-6 days, whereas control grafts developed vascular rejection at 3-4 days (cellular vs. vascular, P<0.03). There was a reduction in preformed antibodies (P<0.03). ST combined with moderate cyclosporine prevented rejection at 9+ and 25 days in sensitized recipients compared with vascular rejection at 0.5-2 days for controls (P<0.07). CONCLUSIONS: ST seems to provide protection against vascular rejection. The cellular rejection seems sensitive to cyclosporine.

Full Text

Duke Authors

Cited Authors

  • Sudan, DL; Radio, SJ; Matamoros, A; Johnson, ND; Shearon, CC; Glogowski, KR; Hill, AC; Goertzen, TC; Fox, IJ; Langnas, AN; Beschorner, WE

Published Date

  • January 27, 2000

Published In

Volume / Issue

  • 69 / 2

Start / End Page

  • 232 - 235

PubMed ID

  • 10670632

International Standard Serial Number (ISSN)

  • 0041-1337

Digital Object Identifier (DOI)

  • 10.1097/00007890-200001270-00007


  • eng

Conference Location

  • United States