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Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids.

Publication ,  Journal Article
Lee, WT; Tamai, H; Cohen, P; Teker, AM; Shu, S
Published in: Arch Otolaryngol Head Neck Surg
June 2008

OBJECTIVE: To investigate the therapeutic efficacy of fused dendritic-tumor cell hybrids against murine squamous cell carcinoma (SCC). DESIGN: Squamous cell carcinoma VII is a poorly immunogenic murine SCC tumor in C3H/HEN (H-2(K)) mice. Subdermal tumors were established by inoculation in the mid abdomen of mice. Tumor diameters were measured with a Vernier caliper and used as an indication of treatment efficacy. Survival studies were performed on mice with 3-day pulmonary metastasis or subdermal tumors. Dendritic cells were generated from bone marrow and cultured for 8 days. Dendritic cells were harvested and mixed with cultured tumor cells in a 1:1 ratio. Cell fusion was achieved by exposing the cell mixture to an alternate electrical current to bring cells into alignment and close together, followed by a short direct electrical current pulse. SUBJECTS: Female C3H/HEN mice aged 8 to 12 weeks. INTERVENTIONS: Mice with 3-day established SCCVII tumors were vaccinated by inguinal intranodal injection of fusion cells (0.3 x 10(6) per side). To support the development of antitumor immunity, mice were given adjuvant injections intraperitoneally. Anti-OX40R monoclonal antibodies or interleukin 12 were used. Treatment groups included no treatment, anti-OX40R monoclonal antibodies or adjuvant IL-12 alone, fusion cells alone, and fusion cells with adjuvant treatment. MAIN OUTCOME MEASURES: Tumor size and overall survival. RESULTS: Mice treated with adjuvant treatment or fusion cells alone did not show a statistical difference in tumor growth when compared with controls. In contrast, fusion cells with adjuvant treatment demonstrated a significant decrease in tumor size when compared with nontreated mice (P < .001). Treatment with fusion cells also resulted in increased survival in the pulmonary metastasis and subdermal tumor models. CONCLUSION: Immunotherapy with fused dendritic-tumor cell hybrids can significantly affect 3-day established sSCC VII tumor growth.

Duke Scholars

Published In

Arch Otolaryngol Head Neck Surg

DOI

EISSN

1538-361X

Publication Date

June 2008

Volume

134

Issue

6

Start / End Page

608 / 613

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Otorhinolaryngology
  • Mice, Inbred C3H
  • Mice
  • Immunotherapy
  • Hybrid Cells
  • Female
  • Disease Models, Animal
  • Dendritic Cells
  • Cell Fusion
 

Citation

APA
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ICMJE
MLA
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Lee, W. T., Tamai, H., Cohen, P., Teker, A. M., & Shu, S. (2008). Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids. Arch Otolaryngol Head Neck Surg, 134(6), 608–613. https://doi.org/10.1001/archotol.134.6.608
Lee, Walter T., Hidemasa Tamai, Peter Cohen, Aysenur Meric Teker, and Suyu Shu. “Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids.Arch Otolaryngol Head Neck Surg 134, no. 6 (June 2008): 608–13. https://doi.org/10.1001/archotol.134.6.608.
Lee WT, Tamai H, Cohen P, Teker AM, Shu S. Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids. Arch Otolaryngol Head Neck Surg. 2008 Jun;134(6):608–13.
Lee, Walter T., et al. “Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids.Arch Otolaryngol Head Neck Surg, vol. 134, no. 6, June 2008, pp. 608–13. Pubmed, doi:10.1001/archotol.134.6.608.
Lee WT, Tamai H, Cohen P, Teker AM, Shu S. Immunotherapy of established murine squamous cell carcinoma using fused dendritic-tumor cell hybrids. Arch Otolaryngol Head Neck Surg. 2008 Jun;134(6):608–613.

Published In

Arch Otolaryngol Head Neck Surg

DOI

EISSN

1538-361X

Publication Date

June 2008

Volume

134

Issue

6

Start / End Page

608 / 613

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Otorhinolaryngology
  • Mice, Inbred C3H
  • Mice
  • Immunotherapy
  • Hybrid Cells
  • Female
  • Disease Models, Animal
  • Dendritic Cells
  • Cell Fusion