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Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments.

Publication ,  Journal Article
Baghian, A; Luftig, M; Black, JB; Meng, YX; Pau, CP; Voss, T; Pellett, PE; Kousoulas, KG
Published in: Virology
March 30, 2000

Human herpesvirus 8 (HHV-8) or Kaposi's sarcoma-associated herpesvirus (KSHV) is the only known human member of the Rhadinovirus genus of the gammaherpesvirus subfamily. Antibodies against peptides representing portions of the amino- and carboxyl-termini of HHV-8 gB were produced and used to detect gB expression in Vero cells transfected with the gB gene, in the HHV-8-harboring cell line, BCBL-1, and in purified virions. Expression of gB was detected in approximately 3% of uninduced BCBL-1 cells, while up to 30% of the cells expressed gB after 12-O-tetradecanoylphorbol-13-acetate (TPA) induction of virus replication. Indirect immunofluorescence assays and confocal microscopy showed that gB was distributed throughout the cytoplasm of BCBL-1 cells and transfected Vero cells. Immunoblot analyses of virion preparations revealed the presence of full-length as well as two smaller than full-length gB-derived species corresponding to the amino- and carboxy-terminal portions of gB, respectively. Biochemical analysis of the gB carbohydrate moieties using glycosylation inhibitors revealed that gB contained N-linked oligosaccharides of the high-mannose type, characteristic of precursor carbohydrate chains added in the endoplasmic reticulum.

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Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 30, 2000

Volume

269

Issue

1

Start / End Page

18 / 25

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Envelope Proteins
  • Vero Cells
  • Tunicamycin
  • Tumor Cells, Cultured
  • Transfection
  • Tetradecanoylphorbol Acetate
  • Protein Processing, Post-Translational
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
 

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Baghian, A., Luftig, M., Black, J. B., Meng, Y. X., Pau, C. P., Voss, T., … Kousoulas, K. G. (2000). Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments. Virology, 269(1), 18–25. https://doi.org/10.1006/viro.2000.0198
Baghian, A., M. Luftig, J. B. Black, Y. X. Meng, C. P. Pau, T. Voss, P. E. Pellett, and K. G. Kousoulas. “Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments.Virology 269, no. 1 (March 30, 2000): 18–25. https://doi.org/10.1006/viro.2000.0198.
Baghian A, Luftig M, Black JB, Meng YX, Pau CP, Voss T, et al. Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments. Virology. 2000 Mar 30;269(1):18–25.
Baghian, A., et al. “Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments.Virology, vol. 269, no. 1, Mar. 2000, pp. 18–25. Pubmed, doi:10.1006/viro.2000.0198.
Baghian A, Luftig M, Black JB, Meng YX, Pau CP, Voss T, Pellett PE, Kousoulas KG. Glycoprotein B of human herpesvirus 8 is a component of the virion in a cleaved form composed of amino- and carboxyl-terminal fragments. Virology. 2000 Mar 30;269(1):18–25.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

March 30, 2000

Volume

269

Issue

1

Start / End Page

18 / 25

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Envelope Proteins
  • Vero Cells
  • Tunicamycin
  • Tumor Cells, Cultured
  • Transfection
  • Tetradecanoylphorbol Acetate
  • Protein Processing, Post-Translational
  • Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase