Patterns of nucleotide variation and reproductive isolation between a Mimulus allotetraploid and its progenitor species.

Published

Journal Article

Here we report our characterization of a widespread, highly selfing Mimulus allotetraploid formed by interspecific hybridization between M. nasutus and M. guttatus. Nucleotide variation at two nuclear loci (mCYCA and mAP3) within and among tetraploid populations resolves two haplotype clusters for each locus: one shares near identity with sequences from M. nasutus and the other group shares substantial variation with M. guttatus. With respect to the two loci studied, each allotetraploid individual is a 'fixed heterozygote' carrying sequences from both clusters. Moreover, mCYCA variation is consistent with at least two evolutionary origins for the Mimulus allotetraploid. We show that the allotetraploid is strongly reproductively isolated from M. nasutus and M. guttatus; interploidy crosses produce almost no viable seeds. By extension, we infer strong triploid block and argue that Mimulus allotetraploid formation might proceed in one step via the union of unreduced gametes in an M. nasutus-M. guttatus F(1) hybrid. We also discuss the potential roles of mating system and flowering asynchrony in allotetraploid establishment.

Full Text

Duke Authors

Cited Authors

  • Sweigart, AL; Martin, NH; Willis, JH

Published Date

  • April 2008

Published In

Volume / Issue

  • 17 / 8

Start / End Page

  • 2089 - 2100

PubMed ID

  • 18312552

Pubmed Central ID

  • 18312552

Electronic International Standard Serial Number (EISSN)

  • 1365-294X

International Standard Serial Number (ISSN)

  • 0962-1083

Digital Object Identifier (DOI)

  • 10.1111/j.1365-294x.2008.03707.x

Language

  • eng