Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.
Publication
, Journal Article
Fierer, N; Jackson, JA; Vilgalys, R; Jackson, RB
Published in: Applied and environmental microbiology
July 2005
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.
Duke Scholars
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Published In
Applied and environmental microbiology
DOI
EISSN
1098-5336
ISSN
0099-2240
Publication Date
July 2005
Volume
71
Issue
7
Start / End Page
4117 / 4120
Related Subject Headings
- Species Specificity
- Soil Microbiology
- Sensitivity and Specificity
- Polymerase Chain Reaction
- Microbiology
- Fungi
- Ecosystem
- DNA, Fungal
- DNA, Bacterial
- DNA Primers
Citation
APA
Chicago
ICMJE
MLA
NLM
Fierer, N., Jackson, J. A., Vilgalys, R., & Jackson, R. B. (2005). Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and Environmental Microbiology, 71(7), 4117–4120. https://doi.org/10.1128/aem.71.7.4117-4120.2005
Fierer, Noah, Jason A. Jackson, Rytas Vilgalys, and Robert B. Jackson. “Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.” Applied and Environmental Microbiology 71, no. 7 (July 2005): 4117–20. https://doi.org/10.1128/aem.71.7.4117-4120.2005.
Fierer N, Jackson JA, Vilgalys R, Jackson RB. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and environmental microbiology. 2005 Jul;71(7):4117–20.
Fierer, Noah, et al. “Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.” Applied and Environmental Microbiology, vol. 71, no. 7, July 2005, pp. 4117–20. Epmc, doi:10.1128/aem.71.7.4117-4120.2005.
Fierer N, Jackson JA, Vilgalys R, Jackson RB. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays. Applied and environmental microbiology. 2005 Jul;71(7):4117–4120.
Published In
Applied and environmental microbiology
DOI
EISSN
1098-5336
ISSN
0099-2240
Publication Date
July 2005
Volume
71
Issue
7
Start / End Page
4117 / 4120
Related Subject Headings
- Species Specificity
- Soil Microbiology
- Sensitivity and Specificity
- Polymerase Chain Reaction
- Microbiology
- Fungi
- Ecosystem
- DNA, Fungal
- DNA, Bacterial
- DNA Primers