Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.


Journal Article

Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.

Full Text

Duke Authors

Cited Authors

  • Fierer, N; Jackson, JA; Vilgalys, R; Jackson, RB

Published Date

  • July 2005

Published In

Volume / Issue

  • 71 / 7

Start / End Page

  • 4117 - 4120

PubMed ID

  • 16000830

Pubmed Central ID

  • 16000830

Electronic International Standard Serial Number (EISSN)

  • 1098-5336

International Standard Serial Number (ISSN)

  • 0099-2240

Digital Object Identifier (DOI)

  • 10.1128/AEM.71.7.4117-4120.2005


  • eng