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Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia.

Publication ,  Journal Article
Bean, SM; Eltoum, I; Horton, DK; Whitlow, L; Chhieng, DC
Published in: Am J Surg Pathol
April 2007

Anal intraepithelial neoplasia (AIN) is a human papilloma virus related lesion. It has been shown that infection with high-risk human papilloma virus results in up-regulation of p16 and increased cellular proliferation. The objective of this study is to correlate p16 expression and cellular proliferation measured by Ki-67 staining with the degree of dysplasia in the anal canal and to determine the efficacy of these markers in diagnosing high-grade AIN. Seventy-five anal specimens from 55 patients (37 men; 18 women; mean age: 48 y; median: 44 y; range 25 to 96 y) were studied including 35 normal/reactive lesions, 23 low-grade AIN (AIN I and condyloma), and 17 high-grade AIN (AIN II and III). Immunostaining for p16 and Ki-67 was performed. Expression of p16 in AIN correlated with that of Ki-67 (P<0.001). High-grade AIN often demonstrated p16 staining in more than one-third of the thickness of the epithelium in a diffuse/continuous fashion. p16 expression in low-grade AIN was often restricted to the lower 1/3 of the epithelium and/or was focal and discontinuous. The expression of both p16 and Ki-67 correlated with the degree of dysplasia (P<0.01). When positive p16 staining was defined as the presence of diffuse/continuous staining in more than one-third of the thickness of epithelium, the sensitivity, specificity, and accuracy of p16 as a marker for diagnosing high-grade AIN were 76%, 86%, and 84%, respectively. When positive Ki-67 staining was defined as the presence of nuclear staining in more than 25% of the cells in more than one-third of the thickness of epithelium, the sensitivity, specificity, and accuracy of Ki-67 as a marker for diagnosing high-grade AIN were 71%, 84%, and 83% respectively. Both p16 and Ki-67 are reliable markers for diagnosing high-grade AIN.

Duke Scholars

Published In

Am J Surg Pathol

DOI

ISSN

0147-5185

Publication Date

April 2007

Volume

31

Issue

4

Start / End Page

555 / 561

Location

United States

Related Subject Headings

  • Tumor Virus Infections
  • Sensitivity and Specificity
  • Precancerous Conditions
  • Pathology
  • Papillomavirus Infections
  • Male
  • Ki-67 Antigen
  • Immunohistochemistry
  • Humans
  • History, 17th Century
 

Citation

APA
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MLA
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Bean, S. M., Eltoum, I., Horton, D. K., Whitlow, L., & Chhieng, D. C. (2007). Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia. Am J Surg Pathol, 31(4), 555–561. https://doi.org/10.1097/PAS.0b013e31802ca3f4
Bean, Sarah M., Isam Eltoum, Debra K. Horton, Leisa Whitlow, and David C. Chhieng. “Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia.Am J Surg Pathol 31, no. 4 (April 2007): 555–61. https://doi.org/10.1097/PAS.0b013e31802ca3f4.
Bean SM, Eltoum I, Horton DK, Whitlow L, Chhieng DC. Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia. Am J Surg Pathol. 2007 Apr;31(4):555–61.
Bean, Sarah M., et al. “Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia.Am J Surg Pathol, vol. 31, no. 4, Apr. 2007, pp. 555–61. Pubmed, doi:10.1097/PAS.0b013e31802ca3f4.
Bean SM, Eltoum I, Horton DK, Whitlow L, Chhieng DC. Immunohistochemical expression of p16 and Ki-67 correlates with degree of anal intraepithelial neoplasia. Am J Surg Pathol. 2007 Apr;31(4):555–561.

Published In

Am J Surg Pathol

DOI

ISSN

0147-5185

Publication Date

April 2007

Volume

31

Issue

4

Start / End Page

555 / 561

Location

United States

Related Subject Headings

  • Tumor Virus Infections
  • Sensitivity and Specificity
  • Precancerous Conditions
  • Pathology
  • Papillomavirus Infections
  • Male
  • Ki-67 Antigen
  • Immunohistochemistry
  • Humans
  • History, 17th Century