Biocatalytic microcontact printing.

Journal Article (Journal Article)

Immobilized biocatalytic lithography is presented as an application of soft lithography. In traditional microcontact printing, diffusion limits resolution of pattern transfer. By using an immobilized catalyst, the lateral resolution of microcontact printing would depend only on the length and flexibility of the tether (<2 nm) as opposed to diffusion (>100 nm). In the work, exonuclease reversibly immobilized on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal fluorescence microscopy to be approximately 70%.

Full Text

Duke Authors

Cited Authors

  • Snyder, PW; Johannes, MS; Vogen, BN; Clark, RL; Toone, EJ

Published Date

  • September 2007

Published In

Volume / Issue

  • 72 / 19

Start / End Page

  • 7459 - 7461

PubMed ID

  • 17705546

Pubmed Central ID

  • 17705546

Electronic International Standard Serial Number (EISSN)

  • 1520-6904

International Standard Serial Number (ISSN)

  • 0022-3263

Digital Object Identifier (DOI)

  • 10.1021/jo0711541

Language

  • eng