Using alpha-helical coiled-coils to design nanostructured metalloporphyrin arrays.

Journal Article (Journal Article)

We have developed a computational design strategy based on the alpha-helical coiled-coil to generate modular peptide motifs capable of assembling into metalloporphyrin arrays of varying lengths. The current study highlights the extension of a two-metalloporphyrin array to a four-metalloporphyrin array through the incorporation of a coiled-coil repeat unit. Molecular dynamics simulations demonstrate that the initial design evolves rapidly to a stable structure with a small rmsd compared to the original model. Biophysical characterization reveals elongated proteins of the desired length, correct cofactor stoichiometry, and cofactor specificity. The successful extension of the two-porphyrin array demonstrates how this methodology serves as a foundation to create linear assemblies of organized electrically and optically responsive cofactors.

Full Text

Duke Authors

Cited Authors

  • McAllister, KA; Zou, H; Cochran, FV; Bender, GM; Senes, A; Fry, HC; Nanda, V; Keenan, PA; Lear, JD; Saven, JG; Therien, MJ; Blasie, JK; DeGrado, WF

Published Date

  • September 2008

Published In

Volume / Issue

  • 130 / 36

Start / End Page

  • 11921 - 11927

PubMed ID

  • 18710226

Pubmed Central ID

  • PMC3663296

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja800697g


  • eng