In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes.


Journal Article

PURPOSE:Noninvasive in vivo cell-tracking techniques are necessary to advance the field of cellular-based therapeutics as well as to elucidate mechanisms governing in vivo cell biology. Fluorescence is commonly used for in vitro and postmortem biomedical studies but has been limited by autofluorescence at the whole-animal level. PROCEDURES:In this report, we demonstrate the ability of in vivo fluorescent lifetime imaging to remove autofluorescence and thereby enable in vivo dendritic cell tracking in naïve mice. Specifically, we track mature dendritic cells (DCs) labeled internally with near-infrared-emissive polymersomes (NIR-DCs). RESULTS:We establish the ability to detect labeled cells in vivo and image NIR-DC trafficking after both intravenous and subcutaneous delivery. In addition, we demonstrate the longitudinal capacity of this method by characterizing NIR-DC migration kinetics in the popliteal lymph node. CONCLUSIONS:This work provides a tool to evaluate dendritic-cell-based immunotherapy and generates novel opportunities for in vivo fluorescence imaging.

Full Text

Duke Authors

Cited Authors

  • Christian, NA; Benencia, F; Milone, MC; Li, G; Frail, PR; Therien, MJ; Coukos, G; Hammer, DA

Published Date

  • May 2009

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 167 - 177

PubMed ID

  • 19194761

Pubmed Central ID

  • 19194761

Electronic International Standard Serial Number (EISSN)

  • 1860-2002

International Standard Serial Number (ISSN)

  • 1536-1632

Digital Object Identifier (DOI)

  • 10.1007/s11307-008-0184-x


  • eng