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In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes.

Publication ,  Journal Article
Christian, NA; Benencia, F; Milone, MC; Li, G; Frail, PR; Therien, MJ; Coukos, G; Hammer, DA
Published in: Molecular imaging and biology
May 2009

Noninvasive in vivo cell-tracking techniques are necessary to advance the field of cellular-based therapeutics as well as to elucidate mechanisms governing in vivo cell biology. Fluorescence is commonly used for in vitro and postmortem biomedical studies but has been limited by autofluorescence at the whole-animal level.In this report, we demonstrate the ability of in vivo fluorescent lifetime imaging to remove autofluorescence and thereby enable in vivo dendritic cell tracking in naïve mice. Specifically, we track mature dendritic cells (DCs) labeled internally with near-infrared-emissive polymersomes (NIR-DCs).We establish the ability to detect labeled cells in vivo and image NIR-DC trafficking after both intravenous and subcutaneous delivery. In addition, we demonstrate the longitudinal capacity of this method by characterizing NIR-DC migration kinetics in the popliteal lymph node.This work provides a tool to evaluate dendritic-cell-based immunotherapy and generates novel opportunities for in vivo fluorescence imaging.

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Published In

Molecular imaging and biology

DOI

EISSN

1860-2002

ISSN

1536-1632

Publication Date

May 2009

Volume

11

Issue

3

Start / End Page

167 / 177

Related Subject Headings

  • Research Design
  • Nuclear Medicine & Medical Imaging
  • Mice
  • Methods
  • Lymph Nodes
  • Kinetics
  • Fluorescence
  • Diagnostic Imaging
  • Dendritic Cells
  • Cytological Techniques
 

Citation

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Christian, N. A., Benencia, F., Milone, M. C., Li, G., Frail, P. R., Therien, M. J., … Hammer, D. A. (2009). In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes. Molecular Imaging and Biology, 11(3), 167–177. https://doi.org/10.1007/s11307-008-0184-x
Christian, Natalie A., Fabian Benencia, Michael C. Milone, Guizhi Li, Paul R. Frail, Michael J. Therien, George Coukos, and Daniel A. Hammer. “In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes.Molecular Imaging and Biology 11, no. 3 (May 2009): 167–77. https://doi.org/10.1007/s11307-008-0184-x.
Christian NA, Benencia F, Milone MC, Li G, Frail PR, Therien MJ, et al. In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes. Molecular imaging and biology. 2009 May;11(3):167–77.
Christian, Natalie A., et al. “In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes.Molecular Imaging and Biology, vol. 11, no. 3, May 2009, pp. 167–77. Epmc, doi:10.1007/s11307-008-0184-x.
Christian NA, Benencia F, Milone MC, Li G, Frail PR, Therien MJ, Coukos G, Hammer DA. In vivo dendritic cell tracking using fluorescence lifetime imaging and near-infrared-emissive polymersomes. Molecular imaging and biology. 2009 May;11(3):167–177.
Journal cover image

Published In

Molecular imaging and biology

DOI

EISSN

1860-2002

ISSN

1536-1632

Publication Date

May 2009

Volume

11

Issue

3

Start / End Page

167 / 177

Related Subject Headings

  • Research Design
  • Nuclear Medicine & Medical Imaging
  • Mice
  • Methods
  • Lymph Nodes
  • Kinetics
  • Fluorescence
  • Diagnostic Imaging
  • Dendritic Cells
  • Cytological Techniques