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Antibodies from patients with heparin-induced thrombocytopenia stimulate monocytic cells to express tissue factor and secrete interleukin-8.

Publication ,  Journal Article
Arepally, GM; Mayer, IM
Published in: Blood
August 15, 2001

Thrombosis is a life-threatening complication that occurs in a subset of patients with heparin-induced thrombocytopenia (HITT). The pathogenic mechanisms underlying the variable occurrence of thrombosis in HITT is poorly understood. It was hypothesized that monocyte activation leading to tissue factor expression may play a role in promoting a thrombogenic state in HITT. This study demonstrates that a human platelet factor 4 (PF4)/heparin-specific murine monoclonal antibody (KKO) binds to peripheral blood-derived human monocytes in a PF4-dependent manner. KKO and antibodies from patients with HITT induce monocytes to synthesize and secrete interleukin-8 and induce cell-surface procoagulant activity, which is abrogated following treatment with antihuman tissue factor antibody. The findings suggest a novel mechanism by which PF4/heparin antibodies may promote a hypercoagulable state in patients with HITT. (Blood. 2001;98:1252-1254)

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Published In

Blood

DOI

ISSN

0006-4971

Publication Date

August 15, 2001

Volume

98

Issue

4

Start / End Page

1252 / 1254

Location

United States

Related Subject Headings

  • Thrombosis
  • Thromboplastin
  • Thrombophilia
  • Thrombocytopenia
  • Platelet Factor 4
  • Monocytes
  • Interleukin-8
  • Immunology
  • Humans
  • Heparin
 

Citation

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Arepally, G. M., & Mayer, I. M. (2001). Antibodies from patients with heparin-induced thrombocytopenia stimulate monocytic cells to express tissue factor and secrete interleukin-8. Blood, 98(4), 1252–1254. https://doi.org/10.1182/blood.v98.4.1252
Arepally, G. M., and I. M. Mayer. “Antibodies from patients with heparin-induced thrombocytopenia stimulate monocytic cells to express tissue factor and secrete interleukin-8.Blood 98, no. 4 (August 15, 2001): 1252–54. https://doi.org/10.1182/blood.v98.4.1252.
Arepally, G. M., and I. M. Mayer. “Antibodies from patients with heparin-induced thrombocytopenia stimulate monocytic cells to express tissue factor and secrete interleukin-8.Blood, vol. 98, no. 4, Aug. 2001, pp. 1252–54. Pubmed, doi:10.1182/blood.v98.4.1252.

Published In

Blood

DOI

ISSN

0006-4971

Publication Date

August 15, 2001

Volume

98

Issue

4

Start / End Page

1252 / 1254

Location

United States

Related Subject Headings

  • Thrombosis
  • Thromboplastin
  • Thrombophilia
  • Thrombocytopenia
  • Platelet Factor 4
  • Monocytes
  • Interleukin-8
  • Immunology
  • Humans
  • Heparin