Straight and curved conformations of FtsZ are regulated by GTP hydrolysis.

Published

Journal Article

FtsZ assembles in vitro into protofilaments that can adopt two conformations-the straight conformation, which can assemble further into two-dimensional protofilament sheets, and the curved conformation, which forms minirings about 23 nm in diameter. Here, we describe the structure of FtsZ tubes, which are a variation of the curved conformation. In the tube the curved protofilament forms a shallow helix with a diameter of 23 nm and a pitch of 18 or 24 degrees. We suggest that this shallow helix is the relaxed structure of the curved protofilament in solution. We provide evidence that GTP favors the straight conformation while GDP favors the curved conformation. In particular, exclusively straight protofilaments and protofilament sheets are assembled in GMPCPP, a nonhydrolyzable GTP analog, or in GTP following chelation of Mg, which blocks GTP hydrolysis. Assembly in GDP produces exclusively tubes. The transition from straight protofilaments to the curved conformation may provide a mechanism whereby the energy of GTP hydrolysis is used to generate force for the constriction of the FtsZ ring in cell division.

Full Text

Duke Authors

Cited Authors

  • Lu, C; Reedy, M; Erickson, HP

Published Date

  • January 2000

Published In

Volume / Issue

  • 182 / 1

Start / End Page

  • 164 - 170

PubMed ID

  • 10613876

Pubmed Central ID

  • 10613876

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.182.1.164-170.2000

Language

  • eng

Conference Location

  • United States