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Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching.

Publication ,  Journal Article
Stricker, J; Maddox, P; Salmon, ED; Erickson, HP
Published in: Proc Natl Acad Sci U S A
March 5, 2002

FtsZ, the major cytoskeletal component of the bacterial cell-division machine, assembles into a ring (the Z-ring) that contracts at septation. FtsZ is a bacterial homolog of tubulin, with similar tertiary structure, GTP hydrolysis, and in vitro assembly. We used green fluorescent protein-labeled FtsZ and fluorescence recovery after photobleaching to show that the E. coli Z-ring is extremely dynamic, continually remodeling itself with a half-time of 30 s. ZipA, a membrane protein involved in cell division that colocalizes with FtsZ, was equally dynamic. The Z-ring of the mutant ftsZ84, which has 1/10 the guanosine triphosphatase activity of wild-type FtsZ in vitro, showed a 9-fold slower turnover in vivo. This finding implies that assembly dynamics are determined primarily by GTP hydrolysis. Despite the greatly reduced assembly dynamics, the ftsZ84 cells divide with a normal cell-cycle time.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 5, 2002

Volume

99

Issue

5

Start / End Page

3171 / 3175

Location

United States

Related Subject Headings

  • Time Factors
  • Recombinant Fusion Proteins
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Fluorescence
  • Escherichia coli Proteins
  • Escherichia coli
  • Cytoskeletal Proteins
  • Cell Cycle Proteins
  • Carrier Proteins
 

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Stricker, J., Maddox, P., Salmon, E. D., & Erickson, H. P. (2002). Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci U S A, 99(5), 3171–3175. https://doi.org/10.1073/pnas.052595099
Stricker, Jesse, Paul Maddox, E. D. Salmon, and Harold P. Erickson. “Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching.Proc Natl Acad Sci U S A 99, no. 5 (March 5, 2002): 3171–75. https://doi.org/10.1073/pnas.052595099.
Stricker J, Maddox P, Salmon ED, Erickson HP. Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3171–5.
Stricker, Jesse, et al. “Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching.Proc Natl Acad Sci U S A, vol. 99, no. 5, Mar. 2002, pp. 3171–75. Pubmed, doi:10.1073/pnas.052595099.
Stricker J, Maddox P, Salmon ED, Erickson HP. Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3171–3175.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 5, 2002

Volume

99

Issue

5

Start / End Page

3171 / 3175

Location

United States

Related Subject Headings

  • Time Factors
  • Recombinant Fusion Proteins
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Fluorescence
  • Escherichia coli Proteins
  • Escherichia coli
  • Cytoskeletal Proteins
  • Cell Cycle Proteins
  • Carrier Proteins