Structural and functional domains of the Drosophila ncd microtubule motor protein.
Journal Article (Journal Article)
Nonclaret disjunctional (ncd) is a kinesin-related microtubule motor protein that is required for proper chromosome distribution in Drosophila. Despite its sequence similarity to kinesin heavy chain, ncd translocates with the opposite polarity as kinesin, toward microtubule minus ends. We have expressed different regions of the protein in bacteria and analyzed the proteins for function. Results indicate that ncd consists of three domains: a basic, proline-rich N-terminal "tail," a central alpha-helical coiled-coil stalk, and a C-terminal motor domain. The ncd N terminus proteins bundle microtubules in motility assays and show ATP-independent binding to microtubules in solution. Truncated proteins, lacking the tail but containing the predicted motor domain and differing lengths of the stalk, did not support microtubule gliding in in vitro assays but showed microtubule-stimulated MgATPase activity in solution. Addition of a nonspecific N terminus to two of the truncated proteins restored directional gliding and rotation of microtubules in motility assays, demonstrating that these properties map to the predicted mechanochemical domain of ncd. Physical properties of the C terminus proteins indicate that the stalk region is important for dimerization and that the ncd protein probably exists and functions as a dimer.
Full Text
Duke Authors
Cited Authors
- Chandra, R; Salmon, ED; Erickson, HP; Lockhart, A; Endow, SA
Published Date
- April 25, 1993
Published In
Volume / Issue
- 268 / 12
Start / End Page
- 9005 - 9013
PubMed ID
- 8473343
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States